Journal article
Sequence-Independent Assembly of Spermatid mRNAs into Messenger Ribonucleoprotein Particles
Molecular and cellular biology, Vol.19(5), pp.3904-3915
05/1999
Handle:
https://hdl.handle.net/2376/105759
PMCID: PMC84248
PMID: 10207114
Abstract
During mammalian spermatogenesis, meiosis is followed by a brief period of high transcriptional activity. At this time a large amount of mRNA is stored as messenger ribonucleoprotein (mRNP) particles. All subsequent processes of sperm maturation occur in the complete absence of transcription, primarily using proteins which are newly synthesized from these stored mRNAs. By expressing transgene mRNAs in the early haploid spermatids of mice, we have investigated the sequence requirements for determining whether specific mRNAs in these cells will be stored as mRNP particles or be assembled into polysomes. The results suggest that mRNAs which are transcribed in spermatids are assembled into mRNP particles by a mechanism that acts independently of mRNA sequence. Our findings reveal a fundamental similarity between the mechanisms of translational control used in spermatogenesis and oogenesis.
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Details
- Title
- Sequence-Independent Assembly of Spermatid mRNAs into Messenger Ribonucleoprotein Particles
- Creators
- Edward E Schmidt - Howard Hughes Medical InstituteEric S Hanson - Howard Hughes Medical InstituteMario R Capecchi - Howard Hughes Medical Institute
- Publication Details
- Molecular and cellular biology, Vol.19(5), pp.3904-3915
- Academic Unit
- UNKNOWN
- Publisher
- American Society for Microbiology
- Identifiers
- 99900546606501842
- Language
- English
- Resource Type
- Journal article