Journal article
Sequence‐Based Introgression Mapping Identifies Candidate White Mold Tolerance Genes in Common Bean
The plant genome, Vol.9(2), pp.1-11
07/2016
Handle:
https://hdl.handle.net/2376/105546
PMID: 27898809
Abstract
Core Ideas
Sequenced‐based introgression mapping rapidly maps QTL to a physical location
A highly polymorphic candidate gene associated with apoptosis maps in the WM7.1 QTL
A highly polymorphic gene associated with effector recognition maps in the WM8.3 QTL
White mold, caused by the necrotrophic fungus Sclerotinia sclerotiorum (Lib.) de Bary, is a major disease of common bean (Phaseolus vulgaris L.). WM7.1 and WM8.3 are two quantitative trait loci (QTL) with major effects on tolerance to the pathogen. Advanced backcross populations segregating individually for either of the two QTL, and a recombinant inbred (RI) population segregating for both QTL were used to fine map and confirm the genetic location of the QTL. The QTL intervals were physically mapped using the reference common bean genome sequence, and the physical intervals for each QTL were further confirmed by sequence‐based introgression mapping. Using whole‐genome sequence data from susceptible and tolerant DNA pools, introgressed regions were identified as those with significantly higher numbers of single‐nucleotide polymorphisms (SNPs) relative to the whole genome. By combining the QTL and SNP data, WM7.1 was located to a 660‐kb region that contained 41 gene models on the proximal end of chromosome Pv07, while the WM8.3 introgression was narrowed to a 1.36‐Mb region containing 70 gene models. The most polymorphic candidate gene in the WM7.1 region encodes a BEACH‐domain protein associated with apoptosis. Within the WM8.3 interval, a receptor‐like protein with the potential to recognize pathogen effectors was the most polymorphic gene. The use of gene and sequence‐based mapping identified two candidate genes whose putative functions are consistent with the current model of Sclerotinia pathogenicity.
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Details
- Title
- Sequence‐Based Introgression Mapping Identifies Candidate White Mold Tolerance Genes in Common Bean
- Creators
- Sujan Mamidi - North Dakota State UnivPhillip N Miklas - USDA–ARS, Grain Legume Genetics and Physiology Research UnitJennifer Trapp - USDA–ARS, Grain Legume Genetics and Physiology Research UnitErin Felicetti - USDA–ARS, Grain Legume Genetics and Physiology Research UnitJane Grimwood - HudsonAlpha Institute for BiotechnologyJeremy Schmutz - HudsonAlpha Institute for BiotechnologyRian Lee - North Dakota State UnivPhillip E McClean - North Dakota State Univ
- Publication Details
- The plant genome, Vol.9(2), pp.1-11
- Academic Unit
- Crop and Soil Sciences, Department of
- Publisher
- Crop Science Society of America
- Number of pages
- 11
- Grant note
- Kirkhouse Trust funded Africa Bean Consortium
- Identifiers
- 99900546976801842
- Language
- English
- Resource Type
- Journal article