Sequential Assembly of Flagellar Radial Spokes

Dennis R Diener; Pinfen Yang; Stefan Geimer; Douglas G Cole; Winfield S Sale; Joel L Rosenbaum

doi:10.1002/cm.20520

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Sequential Assembly of Flagellar Radial Spokes

Journal article

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Sequential Assembly of Flagellar Radial Spokes

Dennis R Diener, Pinfen Yang, Stefan Geimer, Douglas G Cole, Winfield S Sale and Joel L Rosenbaum

Cytoskeleton (Hoboken, N.J.), Vol.68(7), pp.389-400

07/2011

DOI: https://doi.org/10.1002/cm.20520

Handle:

https://hdl.handle.net/2376/103251

PMCID: PMC3789627

PMID: 21692193

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Abstract

RSP3

Flagella

Chlamydomonas

Cilia

The unicellular alga Chlamydomonas can assemble two 10 μm flagella in one hour from proteins synthesized in the cell body. Targeting and transporting these proteins to the flagella are simplified by preassembly of macromolecular complexes in the cell body. Radial spokes are flagellar complexes that are partially assembled in the cell body before entering the flagella. On the axoneme, radial spokes are “T” shaped structures with a head of 5 proteins and a stalk of 18 proteins that sediment together at 20S. In the cell body, radial spokes are partially assembled; about half of the radial spoke proteins (RSPs) form a 12S complex. In mutants lacking a single radial spoke protein, smaller spoke subassemblies were identified. When extracts from two such mutants were mixed in vitro the 12S complex was assembled from several smaller complexes demonstrating that portions of the stepwise assembly of radial spoke assembly can be carried out in vitro to elucidate the order of spoke assembly in the cell body.

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Title: Sequential Assembly of Flagellar Radial Spokes
Creators: Dennis R Diener - Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, Connecticut 06520
Pinfen Yang - Department of Biology, Marquette University, Milwaukee, Wisconsin, 53201
Stefan Geimer - Zellbiologie/Elektronenmikroskopie, Universitaet Bayreuth, 95440 Bayreuth, Germany
Douglas G Cole - Department of Biological Sciences, University of Idaho, Moscow, Idaho 83844
Winfield S Sale - Department of Cell Biology, Emory University, School of Medicine, Atlanta, Georgia 30322
Joel L Rosenbaum - Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, Connecticut 06520
Publication Details: Cytoskeleton (Hoboken, N.J.), Vol.68(7), pp.389-400
Academic Unit: Center for Reproductive Biology
Grant note: R01 GM014642 || GM / National Institute of General Medical Sciences : NIGMS R37 GM051173 || GM / National Institute of General Medical Sciences : NIGMS
Identifiers: 99900546626301842
Language: English
Resource Type: Journal article