Journal article
Serologic responses of cattle and other animals infected with Neospora caninum
American journal of veterinary research, Vol.57(3), pp.329-336
03/1996
Handle:
https://hdl.handle.net/2376/112050
PMID: 8669764
Abstract
To examine cross-reactivity among Neospora caninum and closely-related apicomplexans.
Sera from animals were examined for antibody production to N caninum and cross-reactivity to Toxoplasma gondii.
Cattle were experimentally infected with 3 tissue cyst-forming protozoan parasites N caninum, T gondii, and Sarcocystis sp, and calves were monospecifically inoculated with the intestinal coccidia, Eimeria bovis and Cryptosporidium parvum. Similar studies were done in laboratory rabbits inoculated with N caninum, T gondii, Hammondia hammondi, and Sarcocystis sp. Additionally, sera were obtained from ewes, lambs, goats, sows, cats, rats, and mice inoculated with N caninum tachyzoites.
The indirect fluorescent antibody (IFA) and ELISA antibody tests (cattle only) were used to examine reactivity to N caninum; the modified direct agglutination, Sabin-Feldman dye, and IFA tests were used to evaluate reactivity to T gondii.
Serologic cross-reactivity among N caninum, T gondii, and Sarcocystis sp was none or minimal by the IFA test. There was some reactivity to N caninum by the use of ELISA in cattle inoculated with Sarcocystis sp.
The IFA test for N caninum was specific for the diagnosis of neosporosis in animals.
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Details
- Title
- Serologic responses of cattle and other animals infected with Neospora caninum
- Creators
- J P Dubey - USDA, Livestock and Poultry Sciences Institute, Parasite Biology and Epidemiology Laboratory, Beltsville, MD 20705, USAD S LindsayD S AdamsJ M GayT V BaszlerB L BlagburnP Thulliez
- Publication Details
- American journal of veterinary research, Vol.57(3), pp.329-336
- Academic Unit
- Veterinary Clinical Sciences, Department of; Paul G. Allen School for Global Animal Health
- Publisher
- United States
- Identifiers
- 99900547711501842
- Language
- English
- Resource Type
- Journal article