Journal article
Seroprevalence of Babesia equi among horses in Israel using competitive inhibition ELISA and IFA assays
Veterinary parasitology, Vol.76(4), pp.251-259
1998
Handle:
https://hdl.handle.net/2376/106412
PMID: 9650862
Abstract
Sera from 361 horses were tested by indirect immunofluorescence antibody test (IFA) and by competitive inhibition ELISA (cELISA), to detect antibodies to
Babesia equi. The concordance between the assays was 95.7%. Application of a cutoff based on a calculated percent inhibition of <20% gave a total of 22 discrepant results, while only 8 sera negative by the cELISA were found positive by the IFA when a cutoff of >20% inhibition was used. Approximately one-third of all the horses tested were found serologically positive to
B. equi, with more horses testing positive from northern Israel. Among horses raised with access to pasture there was a significant difference in the percentage of seropositive reactors (76.6% in the north and 20.1% in the central region), compared with horses without access to pasture (14.3 and 10.3%, respectively). Nineteen percent of stallions were found to be positive, which was significantly less than the proportions of seropositive mares and geldings: 38 and 42%, respectively. No significant association was found between the mean age of horses and seroreactivity to
B. equi.
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Details
- Title
- Seroprevalence of Babesia equi among horses in Israel using competitive inhibition ELISA and IFA assays
- Creators
- Varda Shkap - Department of Parasitology, Kimron Veterinary Institute, P.O. Box 12, Bet Dagon 50250, IsraelI Cohen - The University of Pennsylvania, School of Veterinary Medicine, Philadelphia, PA, USAB Leibovitz - Department of Parasitology, Kimron Veterinary Institute, P.O. Box 12, Bet Dagon 50250, IsraelSavitsky - Department of Parasitology, Kimron Veterinary Institute, P.O. Box 12, Bet Dagon 50250, IsraelE Pipano - Department of Parasitology, Kimron Veterinary Institute, P.O. Box 12, Bet Dagon 50250, IsraelG Avni - Koret School of Veterinary Medicine, Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, IsraelS Shofer - The University of Pennsylvania, School of Veterinary Medicine, Philadelphia, PA, USAU Giger - The University of Pennsylvania, School of Veterinary Medicine, Philadelphia, PA, USAL Kappmeyer - Animal Disease Research Unit, ARS, USDA, Washington State University, Pullman, WA 99164-7030, USAD Knowles - Animal Disease Research Unit, ARS, USDA, Washington State University, Pullman, WA 99164-7030, USA
- Publication Details
- Veterinary parasitology, Vol.76(4), pp.251-259
- Academic Unit
- Veterinary Microbiology and Pathology, Department of
- Publisher
- Elsevier B.V
- Identifiers
- 99900547072401842
- Language
- English
- Resource Type
- Journal article