Journal article
Sertoli Cells Synthesize and Secrete a Ceruloplasmin-Like Protein
Biology of reproduction, Vol.28(5), pp.1225-1229
06/1983
PMID: 6871315
Abstract
Sertoli cells synthesize and secrete a ceruloplasmin-like protein (testicular ceruloplasmin) that is immunologically similar to serum ceruloplasmin. Rat serum ceruloplasmin was purified and an antiserum was produced to the purified protein which specifically immunoprecipitated a 130,000 dalton protein from rat serum. This ceruloplasmin antiserum was found to also immunoprecipitate a 130,000 dalton protein synthesized and secreted by Sertoli cells. The presence of a protease inhibitor, phenylmethylsulfonyl fluoride (PMSF), was required during the immunoprecipitation procedure to prevent the proteolytic degradation of testicular ceruloplasmin. Immunoprecipitation of proteins secreted by Sertoli cells with an antiserum to rat serum proteins was found to precipitate two proteins, testicular ceruloplasmin and testicular transferrin.
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Details
- Title
- Sertoli Cells Synthesize and Secrete a Ceruloplasmin-Like Protein
- Creators
- Michael K. Skinner - Washington State UniversityMichael D. Griswold - Washington State University
- Publication Details
- Biology of reproduction, Vol.28(5), pp.1225-1229
- Academic Unit
- Biological Sciences, School of
- Identifiers
- 99901080308701842
- Language
- English
- Resource Type
- Journal article