Journal article
Sertoli cells synthesize and secrete transferrin-like protein
The Journal of biological chemistry, Vol.255(20), pp.9523-9525
10/25/1980
Handle:
https://hdl.handle.net/2376/115850
PMID: 6776100
Abstract
One of the major proteins secreted by Sertoli cells (Band 3) has a molecular weight on sodium dodecyl sulfate-polyacrylamide gels which corresponds to rat serum transferrin. The preparation of Sertoli cell-secreted proteins contains an iron-binding protein with electrophoretic properties on native polyacrylamide gels similar to serum transferrin. Antibodies against Sertoli cell-secreted proteins contain a component which will immunoprecipitate serum transferrin. Antibodies to serum transferrin cross-react with and immunoprecipitate a major component (Band 3) of [35S]-methionine-labeled Sertoli cell-secreted proteins. We propose that testicular transferrin is a major secretory product of Sertoli cells in culture. In addition to transferrin, antiserum to rat serum proteins will immunoprecipitate one other polypeptide from preparations of Sertoli cell-secreted proteins.
Metrics
1 File views/ downloads
8 Record Views
Details
- Title
- Sertoli cells synthesize and secrete transferrin-like protein
- Creators
- M K SkinnerM D Griswold
- Publication Details
- The Journal of biological chemistry, Vol.255(20), pp.9523-9525
- Academic Unit
- Biological Sciences, School of
- Publisher
- United States
- Grant note
- HD 00253 / NICHD NIH HHS HD 10808 / NICHD NIH HHS
- Identifiers
- 99900547566901842
- Language
- English
- Resource Type
- Journal article