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Sex-specific differences in transcriptome profiles of brain and muscle tissue of the tropical gar956.80 kB
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Journal article
Sex-specific differences in transcriptome profiles of brain and muscle tissue of the tropical gar
BMC genomics, Vol.18(1), pp.283-283
04/07/2017
Handle:
https://hdl.handle.net/2376/108820
PMCID: PMC5383948
PMID: 28388875
Abstract
Background: The tropical gar (Atractosteus tropicus) is the southernmost species of the seven extant species of gar fishes in the world. In Mexico and Central America, the species is an important food source due to its nutritional quality and low price. Despite its regional importance and increasing concerns about overexploitation and habitat degradation, basic genetic information on the tropical gar is lacking. Determining genetic information on the tropical gar is important for the sustainable management of wild populations, implementation of best practices in aquaculture settings, evolutionary studies of ancient lineages, and an understanding of sex-specific gene expression. In this study, the transcriptome of the tropical gar was sequenced and assembled de novo using tissues from three males and three females using Illumina sequencing technology. Sex-specific and highly differentially expressed transcripts in brain and muscle tissues between adult males and females were subsequently identified. Results: The transcriptome was assembled de novo resulting in 80,611 transcripts with a contig N50 of 3,355 base pairs and over 168 kilobases in total length. Male muscle, brain, and gonad as well as female muscle and brain were included in the assembly. The assembled transcriptome was annotated to identify the putative function of expressed transcripts using Trinotate and SwissProt, a database of well-annotated proteins. The brain and muscle datasets were then aligned to the assembled transcriptome to identify transcripts that were differentially expressed between males and females. The contrast between male and female brain identified 109 transcripts from 106 genes that were significantly differentially expressed. In the muscle comparison, 82 transcripts from 80 genes were identified with evidence for significant differential expression. Almost all genes identified as differentially expressed were sex-specific. The differentially expressed transcripts were enriched for genes involved in cellular functioning, signaling, immune response, and tissue-specific functions.
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Details
- Title
- Sex-specific differences in transcriptome profiles of brain and muscle tissue of the tropical gar
- Creators
- Kayla M Cribbin - Pullman, WA 99164 USACorey R Quackenbush - Pullman, WA 99164 USAKyle Taylor - Pullman, WA 99164 USALenin Arias-Rodriguez - C.P. 86150, Villahermosa, Tabasco MexicoJoanna L Kelley - Pullman, WA 99164 USA
- Publication Details
- BMC genomics, Vol.18(1), pp.283-283
- Academic Unit
- Kelley Lab; Biological Sciences, School of
- Publisher
- BioMed Central; London
- Grant note
- ; IRTA / ;
- Identifiers
- 99900547141701842
- Language
- English
- Resource Type
- Journal article