Journal article
Sex-specific transcriptional responses of the zebrafish (Danio rerio) brain selenoproteome to acute sodium selenite supplementation
Physiological genomics, Vol.45(15), pp.653-666
08/01/2013
Handle:
https://hdl.handle.net/2376/110356
PMCID: PMC3742966
PMID: 23737534
Abstract
The potential benefits of selenium (Se) supplementation are currently under investigation for prevention of certain cancers and treatment of neurological disorders. However, little is known concerning the response of the brain to increased dietary Se under conditions of Se sufficiency, despite the majority of Se supplementation trials occurring in healthy, Se sufficient subjects. We evaluated the transcriptional response of Se-dependent genes, selenoproteins and the genes necessary for their synthesis (the selenoproteome), in the zebrafish (
Danio rerio
) brain to supplementation with nutritionally relevant levels of dietary Se (sodium selenite) during conditions of assumed Se sufficiency. We first used a microarray approach to analyze the response of the brain selenoproteome to dietary Se supplementation for 14 days and then assessed the immediacy and time-scale transcriptional response of the brain selenoproteome to 1, 7, and 14 days of Se supplementation by quantitative real-time PCR (qRT-PCR). The microarray approach did not indicate large-scale influences of Se on the brain transcriptome as a whole or the selenoproteome specifically; only one nonselenoproteome gene (si:ch73-44m9.2) was significantly differentially expressed. Our qRT-PCR results, however, indicate that increases of dietary Se cause small, but significant transcriptional changes within the brain selenoproteome, even after only 1 day of supplementation. These responses were dynamic over a short period of supplementation in a manner highly dependent on sex and the duration of Se supplementation. In nutritional intervention studies, it may be necessary to utilize methods such as qRT-PCR, which allow larger sample sizes, for detecting subtle transcriptional changes in the brain.
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Details
- Title
- Sex-specific transcriptional responses of the zebrafish (Danio rerio) brain selenoproteome to acute sodium selenite supplementation
- Creators
- Maia J Benner - Department of Biological Sciences, University of Idaho, Moscow, IdahoMatt L Settles - Institute for Bioinformatics and Evolutionary Studies, University of Idaho, Moscow, IdahoGordon K Murdoch - Department of Animal and Veterinary Sciences, Moscow, Idaho; andRonald W Hardy - Aquaculture Research Institute, Hagerman Fish Culture Experiment Station, University of Idaho, Hagerman, IdahoBarrie D Robison - Department of Biological Sciences, University of Idaho, Moscow, Idaho
- Publication Details
- Physiological genomics, Vol.45(15), pp.653-666
- Academic Unit
- Animal Sciences, Department of
- Publisher
- American Physiological Society; Bethesda, MD
- Identifiers
- 99900547030501842
- Language
- English
- Resource Type
- Journal article