Journal article
Sexually Dimorphic Effects of Ancestral Exposure to Vinclozolin on Stress Reactivity in Rats
Endocrinology (Philadelphia), Vol.155(10), pp.3853-3866
10/01/2014
Handle:
https://hdl.handle.net/2376/105976
PMCID: PMC4164929
PMID: 25051444
Abstract
How an individual responds to the environment depends upon both personal life history as well as inherited genetic and epigenetic factors from ancestors. Using a 2-hit, 3 generations apart model, we tested how F3 descendants of rats given in utero exposure to the environmental endocrine-disrupting chemical (EDC) vinclozolin reacted to stress during adolescence in their own lives, focusing on sexually dimorphic phenotypic outcomes. In adulthood, male and female F3 vinclozolin-or vehicle-lineage rats, stressed or nonstressed, were behaviorally characterized on a battery of tests and then euthanized. Serum was used for hormone assays, and brains were used for quantitative PCR and transcriptome analyses. Results showed that the effects of ancestral exposure to vinclozolin converged with stress experienced during adolescence in a sexually dimorphic manner. Debilitating effects were seen at all levels of the phenotype, including physiology, behavior, brain metabolism, gene expression, and genome-wide transcriptome modifications in specific brain nuclei. Additionally, females were significantly more vulnerable than males to transgenerational effects of vinclozolin on anxiety but not sociality tests. This fundamental transformation occurs in a manner not predicted by the ancestral exposure or the proximate effects of stress during adolescence, an interaction we refer to as synchronicity.
Metrics
7 Record Views
Details
- Title
- Sexually Dimorphic Effects of Ancestral Exposure to Vinclozolin on Stress Reactivity in Rats
- Creators
- Ross Gillette - Institute for Cellular and Molecular Biology (R.G., I.M.-C., A.C.G., D.C.), The University of Texas at Austin, Austin, Texas 78712Isaac Miller-Crews - Institute for Cellular and Molecular Biology (R.G., I.M.-C., A.C.G., D.C.), The University of Texas at Austin, Austin, Texas 78712Eric E Nilsson - Center for Reproductive Biology (E.E.N., M.K.S.), School of Biological Sciences, Washington State University, Pullman, Washington 99164Michael K Skinner - Center for Reproductive Biology (E.E.N., M.K.S.), School of Biological Sciences, Washington State University, Pullman, Washington 99164Andrea C Gore - Institute for Cellular and Molecular Biology (R.G., I.M.-C., A.C.G., D.C.), The University of Texas at Austin, Austin, Texas 78712, Division of Pharmacology and Toxicology (A.C.G., D.C.), The University of Texas at Austin, Austin, Texas 78712David Crews - Institute for Cellular and Molecular Biology (R.G., I.M.-C., A.C.G., D.C.), The University of Texas at Austin, Austin, Texas 78712, Division of Pharmacology and Toxicology (A.C.G., D.C.), The University of Texas at Austin, Austin, Texas 78712, Department of Integrative Biology (D.C.), The University of Texas at Austin, Austin, Texas 78712
- Publication Details
- Endocrinology (Philadelphia), Vol.155(10), pp.3853-3866
- Academic Unit
- Biological Sciences, School of
- Publisher
- Endocrine Soc
- Number of pages
- 14
- Grant note
- R21MH068273 / NATIONAL INSTITUTE OF MENTAL HEALTH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Mental Health (NIMH) ES020662; ES012974; ES017538; ES023254 / National Institutes of Health; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA National Science Foundation; National Science Foundation (NSF) R21ES017538 / NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Environmental Health Sciences (NIEHS)
- Identifiers
- 99900546543201842
- Language
- English
- Resource Type
- Journal article