SiO2 and ZnO dopants in three-dimensionally printed tricalcium phosphate bone tissue engineering scaffolds enhance osteogenesis and angiogenesis in vivo

Gary Fielding; Susmita Bose

doi:10.1016/j.actbio.2013.07.009

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SiO2 and ZnO dopants in three-dimensionally printed tricalcium phosphate bone tissue engineering scaffolds enhance osteogenesis and angiogenesis in vivo

Journal article

Open access

Peer reviewed

SiO2 and ZnO dopants in three-dimensionally printed tricalcium phosphate bone tissue engineering scaffolds enhance osteogenesis and angiogenesis in vivo

Gary Fielding and Susmita Bose

Acta biomaterialia, Vol.9(11), pp.9137-9148

11/2013

DOI: https://doi.org/10.1016/j.actbio.2013.07.009

Handle:

https://hdl.handle.net/2376/105927

PMID: 23871941

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Abstract

Neovascularization, Physiologic - drug effects

Blood Vessels - growth & development

Tartrate-Resistant Acid Phosphatase

Blood Vessels - ultrastructure

Bone and Bones - drug effects

Tissue Scaffolds - chemistry

Acid Phosphatase - metabolism

Isoenzymes - metabolism

Calcium Phosphates - pharmacology

Printing

Silicon Dioxide - pharmacology

Bone and Bones - physiology

Collagen Type I - metabolism

Tissue Engineering - methods

Implants, Experimental

Osteocalcin - metabolism

Ions - urine

Osteogenesis - drug effects

Rats

Zinc Oxide - pharmacology

Osteoclasts - metabolism

Rats, Sprague-Dawley

Animals

Blood Vessels - drug effects

Mice

Osteoclasts - drug effects

Calcium phosphate (CaP) scaffolds with three-dimensionally-interconnected pores play an important role in mechanical interlocking and biological fixation in bone implant applications. CaPs alone, however, are only osteoconductive (able to guide bone growth). Much attention has been given to the incorporation of biologics and pharmacologics to add osteoinductive (able to cause new bone growth) properties to CaP materials. Because biologics and pharmacologics are generally delicate compounds and also subject to increased regulatory scrutiny, there is a need to investigate alternative methods to introduce osteoinductivity to CaP materials. In this study silica (SiO2) and zinc oxide (ZnO) have been incorporated into three-dimensional printed β-tricalcium phosphate (β-TCP) scaffolds to investigate their potential to trigger osteoinduction in vivo. Silicon and zinc are trace elements that are common in bone and have also been shown to have many beneficial properties, from increased bone regeneration to angiogenesis. Implants were placed in bicortical femur defects introduced to a murine model for up to 16 weeks. The addition of dopants into TCP increased the capacity for new early bone formation by modulating collagen I production and osteocalcin production. Neovascularization was found to be up to three times more than the pure TCP control group. The findings from this study indicate that the combination of SiO2 and ZnO dopants in TCP may be a viable alternative to introducing osteoinductive properties to CaPs.

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Title: SiO2 and ZnO dopants in three-dimensionally printed tricalcium phosphate bone tissue engineering scaffolds enhance osteogenesis and angiogenesis in vivo
Creators: Gary Fielding - W.M. Keck Biomedical Materials Research Laboratory, School of Mechanical and Materials Engineering, Washington State University, Pullman, WA 99164-2920, USA
Susmita Bose
Publication Details: Acta biomaterialia, Vol.9(11), pp.9137-9148
Academic Unit: Mechanical and Materials Engineering, School of
Publisher: England
Grant note: R01 EB007351 / NIBIB NIH HHS R01-EB-007351 / NIBIB NIH HHS
Identifiers: 99900546807601842
Language: English
Resource Type: Journal article