Journal article
Significant associations of stearoyl-CoA desaturase (SCD1) gene with fat deposition and composition in skeletal muscle
International journal of biological sciences, Vol.4(6), pp.345-351
09/25/2008
Handle:
https://hdl.handle.net/2376/113755
PMCID: PMC2556050
PMID: 18825276
Abstract
Gene expression studies in humans and animals have shown that elevated stearoyl-CoA desaturase (SCD1) activity is associated with increased fat accumulation and monounsaturation of saturated fatty acids in skeletal muscle. However, results of the two reported association studies in humans are inconsistent. In the present study, we annotated the bovine
SCD1
gene and identified 3 single nucleotide polymorphisms (SNPs) in its 3'untranslated region (UTR). Genotyping these SNPs on a Wagyu x Limousin reference population revealed that the
SCD1
gene was significantly associated with six fat deposition and fatty acid composition traits in skeletal muscle, but not with subcutaneous fat depth and percent kidney-pelvic-heart fat. In particular, we confirmed that the high stearoyl-CoA desaturase activities/alleles were positively correlated with beef marbling score, amount of monounsaturated fatty acids and conjugated linoleic acid content, but negatively with amount of saturated fatty acids. The inconsistent associations between human studies might be caused by using different sets of markers because we observed that most associated markers are located near the end of 3'UTR. We found that the proximity of the polyadenylation signal site is highly conserved among human, cattle and pig, indicating that the region might contain functional elements involved in posttranscriptional control of
SCD1
activity. In conclusion, our cross species study provided solid evidence to support
SCD1
gene as a critical player in skeletal muscle fat metabolism.
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Details
- Title
- Significant associations of stearoyl-CoA desaturase (SCD1) gene with fat deposition and composition in skeletal muscle
- Creators
- Zhihua Jiang - 1. Department of Animal Sciences, Washington State University, Pullman, WA 99164-6351, USAJennifer J Michal - 1. Department of Animal Sciences, Washington State University, Pullman, WA 99164-6351, USADavid J Tobey - 1. Department of Animal Sciences, Washington State University, Pullman, WA 99164-6351, USATyler F Daniels - 1. Department of Animal Sciences, Washington State University, Pullman, WA 99164-6351, USADaniel C Rule - 2. Department of Animal Science, University of Wyoming, Laramie, WY 82071-3684, USAMichael D MacNeil - 3. USDA-ARS, Fort Keogh Livestock and Range Research Laboratory, Miles City, MT 59301, USA
- Publication Details
- International journal of biological sciences, Vol.4(6), pp.345-351
- Academic Unit
- Animal Sciences, Department of
- Publisher
- Ivyspring International Publisher; Sydney
- Identifiers
- 99900547904201842
- Language
- English
- Resource Type
- Journal article