Silver doped resorbable tricalcium phosphate scaffolds for bone graft applications

Sean Hoover; Solaiman Tarafder; Amit Bandyopadhyay; Susmita Bose

doi:10.1016/j.msec.2017.04.132

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Silver doped resorbable tricalcium phosphate scaffolds for bone graft applications

Journal article

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Silver doped resorbable tricalcium phosphate scaffolds for bone graft applications

Sean Hoover, Solaiman Tarafder, Amit Bandyopadhyay and Susmita Bose

Materials science & engineering. C, Materials for biological applications, Vol.79, pp.763-769

10/01/2017

DOI: https://doi.org/10.1016/j.msec.2017.04.132

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https://hdl.handle.net/2376/108609

PMCID: PMC5609511

PMID: 28629079

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https://doi.org/10.1016/j.msec.2017.04.132View

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Abstract

Tissue Scaffolds

Calcium Phosphates

Porosity

Humans

Silver - chemistry

Tissue Engineering

Bone graft procedures, in particular maxillofacial repair, account for half of the orthopedic procedures done in the US each year. Infection is a major issue in surgery, and should be of primary concern when engineering biomaterials. Silver is of renewed importance today, as it has the ability to potentiate antibiotics against resistant bacterial strains. In order to reduce long term infection risks, it is necessary for the scaffold to maintain a silver ion release for the length of the healing process. In this study, silver doped porous β-tricalcium phosphate (β-TCP) scaffolds were engineered using liquid porogen based method with the goal of meeting these requirements. Silver was added to the β-TCP at three different dopant levels: 0.5wt% Ag O, 1wt% Ag O and 2wt% Ag O. Immersion in pH5 acetate buffer over a 60day period resulted in a total cumulative ion release between 32 and 54μM for dense control scaffolds, and between 80 and 90μM for porous scaffolds. Porosity increased the dissolution rate of the scaffolds by a factor of 2. Human osteoblast cell lines were grown on the scaffolds to measure cytotoxicity and cell proliferation. Porosity increased osteoconduction by doubling the cell growth, and there was no significant cytotoxic effect even for the 2wt% Ag O, as cells were observed on all the samples. Our results showed that silver can be released over a long period without compromising the biocompatibility of the scaffolds.

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Title: Silver doped resorbable tricalcium phosphate scaffolds for bone graft applications
Creators: Sean Hoover - W. M. Keck Biomedical Materials Research Laboratory, School of Mechanical and Materials Engineering, Washington State University, Pullman, WA 99164, USA
Solaiman Tarafder - W. M. Keck Biomedical Materials Research Laboratory, School of Mechanical and Materials Engineering, Washington State University, Pullman, WA 99164, USA
Amit Bandyopadhyay - W. M. Keck Biomedical Materials Research Laboratory, School of Mechanical and Materials Engineering, Washington State University, Pullman, WA 99164, USA
Susmita Bose - W. M. Keck Biomedical Materials Research Laboratory, School of Mechanical and Materials Engineering, Washington State University, Pullman, WA 99164, USA. Electronic address: sbose@wsu.edu
Publication Details: Materials science & engineering. C, Materials for biological applications, Vol.79, pp.763-769
Academic Unit: Mechanical and Materials Engineering, School of
Publisher: Netherlands
Grant note: R01 AR066361 / NIAMS NIH HHS R01 EB007351 / NIBIB NIH HHS
Identifiers: 99900547081601842
Language: English
Resource Type: Journal article