Journal article
Similar nucleotide excision repair capacity in melanocytes and melanoma cells
Cancer research (Chicago, Ill.), Vol.70(12), pp.4922-4930
06/15/2010
Handle:
https://hdl.handle.net/2376/115821
PMCID: PMC2891231
PMID: 20501836
Abstract
Sunlight UV exposure produces DNA photoproducts in skin that are repaired solely by nucleotide excision repair in humans. A significant fraction of melanomas are thought to result from UV-induced DNA damage that escapes repair, however, little evidence is available regarding the functional capacity of normal human melanocytes, malignant melanoma cells and metastatic melanoma cells to repair UV-induced photoproducts in DNA. In this study, we measured nucleotide excision repair in both normal melanocytes and a panel of melanoma cell lines. Our results show that in 11 of 12 melanoma cell lines tested, UV photoproduct repair occurred as efficiently as in primary melanocytes. Importantly, repair capacity was not affected by mutation in the N-Ras or B-Raf oncogenes, nor was a difference observed between a highly metastatic melanoma cell line (A375SM) or its parental line (A375P). Lastly, we found that while p53 status contributed to photoproduct removal efficiency its role did not appear to be mediated by enhanced expression or activity of DNA binding protein DDB2. We concluded that melanoma cells retain capacity for nucleotide excision repair, the loss of which probably does not commonly contribute to melanoma progression.
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Details
- Title
- Similar nucleotide excision repair capacity in melanocytes and melanoma cells
- Creators
- Shobhan Gaddameedhi - Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill, NC, USAMichael G Kemp - Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill, NC, USAJoyce T Reardon - Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill, NC, USAJaniel M Shields - Department of Radiation Oncology, University of North Carolina School of Medicine, Chapel Hill, NC, USAStephanie L Smith-Roe - Department of Pathology and Laboratory Medicine, University of North Carolina School of Medicine, Chapel Hill, NC, USAWilliam K Kaufmann - Department of Pathology and Laboratory Medicine, University of North Carolina School of Medicine, Chapel Hill, NC, USAAziz Sancar - Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill, NC, USA
- Publication Details
- Cancer research (Chicago, Ill.), Vol.70(12), pp.4922-4930
- Academic Unit
- UNKNOWN
- Identifiers
- 99900547897401842
- Language
- English
- Resource Type
- Journal article