Simultaneous detection of two herbicides in fruits and vegetables with nanoparticle-linked immunosorbent and lateral flow immunoassays

Eunice Y. Kwon; Xiaofan Ruan; Fei Yu; Yuehe Lin; Dan Du; Bernard J. Van Wie

doi:10.1016/j.foodchem.2022.133955

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Simultaneous detection of two herbicides in fruits and vegetables with nanoparticle-linked immunosorbent and lateral flow immunoassays

Journal article

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Simultaneous detection of two herbicides in fruits and vegetables with nanoparticle-linked immunosorbent and lateral flow immunoassays

Eunice Y. Kwon, Xiaofan Ruan, Fei Yu, Yuehe Lin, Dan Du and Bernard J. Van Wie

Food chemistry, Vol.399, pp.133955-341265

01/15/2023

DOI: https://doi.org/10.1016/j.foodchem.2022.133955

PMID: 36041336

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Abstract

Fruits and vegetables

Lateral flow immunoassay

Nanoparticle-linked immunosorbent assay

Nanoparticle-mediated differential pulse voltammetry

Peroxidase-like nanoparticles

Herbicides

•Peroxidase-like Pd@Pt nanoparticles are useful in two immunoassay formats.•Atrazine and acetochlor are quantifiable in juices, fruits, and vegetable samples.•Two nanoparticle-amplified immunoassays are validated using HPLC. Herbicides atrazine and acetochlor are used in crop production. Because of environmental and health hazards with respective maximum contamination levels of 3 and 20 ng/mL, quantifying these herbicides is important when considering presence in foods and vegetables. We utilized two Pd@Pt nanoparticle-amplified immunoassays, a colorimetric Pd@Pt nanoparticle-linked immunosorbent assay (NLISA) and differential pulse voltammetry (DPV) dependent on catalytic activity of Pd@Pt in a dual-lateral flow immunoassay (dual-LFIA-DPV). We achieved overall recoveries of 88.5–114 % in juice, fruit, and vegetable samples for both immunoassays. The NLISA yielded limits of detection (LODs) of 0.59 and 0.31 µg/kg and the dual-LFIA-DPV 0.27 and 0.51 µg/kg for the two respective species. Results for both immunoassays were validated by high-performance liquid chromatography (HPLC), for all food and drink samples though LODs are compromised when configuring the HPLC for both species with the same chromatogram. We expect Pd@Pt-based immunoassays to prove useful in various fields.

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Title: Simultaneous detection of two herbicides in fruits and vegetables with nanoparticle-linked immunosorbent and lateral flow immunoassays
Creators: Eunice Y. Kwon - Washington State University
Xiaofan Ruan - Washington State University
Fei Yu - Washington State University
Yuehe Lin - Washington State University
Dan Du - Washington State University
Bernard J. Van Wie - Washington State University
Publication Details: Food chemistry, Vol.399, pp.133955-341265
Academic Unit: School of Mechanical and Materials Engineering
Publisher: Elsevier Ltd
Identifiers: 99901227639501842
Language: English
Resource Type: Journal article