Journal article
Simultaneous production of nisin and lactic acid from cheese whey: Optimization of fermentation conditions through statistically based experimental designs
Applied biochemistry and biotechnology, Vol.114(1), pp.627-638
03/2004
Handle:
https://hdl.handle.net/2376/105099
PMID: 15054282
Abstract
A biorefinery process that utilizes cheese whey as substrate to simultaneously produce nisin, a natural food preservative, and lactic acid, a raw material for biopolymer production, was studied. The conditions for nisin biosynthesis and lactic acid coproduction by Lactococcus lactis subsp. lactis (ATCC 11454) in a whey-based medium were optimized using statistically based experimental designs. A Plackett-Burman design was applied to screen seven parameters for significant factors for the production of nisin and lactic acid. Nutrient supplements, including yeast extract, MgSO4, and KH2PO4, were found to be the significant factors affecting nisin and lactic acid formation. As a follow-up, a central-composite design was applied to optimize these factors. Second-order polynomial models were developed to quantify the relationship between nisin and lactic acid production and the variables. The optimal values of these variables were also determined. Finally, a verification experiment was performed to confirm the optimal values that were predicted by the models. The experimented results agreed well with the model prediction, giving a similar production of 19.3 g/L of lactic acid and 92.9 mg/L of nisin.
Metrics
6 Record Views
Details
- Title
- Simultaneous production of nisin and lactic acid from cheese whey
- Creators
- Chuanbin Liu - Department of Biological Systems Engineering Washington State University 99164-6120 Pullman WAYan Liu - Department of Biological Systems Engineering Washington State University 99164-6120 Pullman WAWei Liao - Department of Biological Systems Engineering Washington State University 99164-6120 Pullman WAZhiyou Wen - Department of Biological Systems Engineering Washington State University 99164-6120 Pullman WAShulin Chen - Department of Biological Systems Engineering Washington State University 99164-6120 Pullman WA
- Publication Details
- Applied biochemistry and biotechnology, Vol.114(1), pp.627-638
- Academic Unit
- Biological Systems Engineering, Department of; Plant Pathology, Department of
- Publisher
- Humana Press; Totowa
- Identifiers
- 99900546779501842
- Language
- English
- Resource Type
- Journal article