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CC BY V4.0, Open Access
Journal article
Single-Atomic Site Catalyst Enhanced Lateral Flow Immunoassay for Point-of-Care Detection of Herbicide
RESEARCH, Vol.2022(1), p.9823290
2022
PMID: 36082212
Abstract
Point-of-care (POC) detection of herbicides is of great importance due to their impact on the environment and potential risks to human health. Here, we design a single-atomic site catalyst (SASC) with excellent peroxidase-like (POD-like) catalytic activity, which enhances the detection performance of corresponding lateral flow immunoassay (LFIA). The iron single-atomic site catalyst (Fe-SASC) is synthesized from hemin-doped ZIF-8, creating active sites that mimic the Fe active center coordination environment of natural enzyme and their functions. Due to its atomically dispersed iron active sites that result in maximum utilization of active metal atoms, the Fe-SASC exhibits superior POD-like activity, which has great potential to replace its natural counterparts. Also, the catalytic mechanism of Fe-SASC is systematically investigated. Utilizing its outstanding catalytic activity, the Fe-SASC is used as label to construct LFIA (Fe-SASC-LFIA) for herbicide detection. The 2,4-dichlorophenoxyacetic acid (2,4-D) is selected as a target here, since it is a commonly used herbicide as well as a biomarker for herbicide exposure evaluation. A linear detection range of 1-250 ng/mL with a low limit of detection (LOD) of 0.82 ng/mL has been achieved. Meanwhile, excellent specificity and selectivity towards 2,4-D have been obtained. The outstanding detection performance of the Fe-SASC-LFIA has also been demonstrated in the detection of human urine samples, indicating the practicability of this POC detection platform for analyzing the 2,4-D exposure level of a person. We believe this proposed Fe-SASC-LFIA has potential as a portable, rapid, and high-sensitive POC detection strategy for pesticide exposure evaluation.
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Details
- Title
- Single-Atomic Site Catalyst Enhanced Lateral Flow Immunoassay for Point-of-Care Detection of Herbicide
- Creators
- Zhaoyuan Lyu - Washington State UniversityShichao Ding - Washington State UniversityPeter Tieu - University of California, IrvineLingzhe Fang - Northern Illinois UniversityXin Li - Washington State UniversityTao Li - Northern Illinois UniversityXiaoqing Pan - University of California, IrvineMark H. Engelhard - Pacific Northwest National LaboratoryXiaofan Ruan - Washington State UniversityDan Du - Washington State UniversitySuiqiong Li - DL ADV Tech, Pullman, WA 99163 USAYuehe Lin - Washington State University
- Publication Details
- RESEARCH, Vol.2022(1), p.9823290
- Academic Unit
- School of Mechanical and Materials Engineering
- Publisher
- Amer Assoc Advancement Science
- Number of pages
- 12
- Grant note
- DE-AC02-06CH11357 / DOE Office of Science by Argonne National Laboratory; United States Department of Energy (DOE) 1R43ES031885-01 / National Institute of Environmental Health Sciences of the US National Institutes of Health; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Environmental Health Sciences (NIEHS)
- Identifiers
- 99901227639901842
- Language
- English
- Resource Type
- Journal article