Files and links (2)
Single-cell RNA-seq uncovers dynamic processes and critical regulators in mouse spermatogenesis6.27 MB
CC BY V4.0, Open Access
Journal article
Single-cell RNA-seq uncovers dynamic processes and critical regulators in mouse spermatogenesis
Cell research, Vol.28(9), pp.879-896
09/2018
Handle:
https://hdl.handle.net/2376/114943
PMCID: PMC6123400
PMID: 30061742
Abstract
A systematic interrogation of male germ cells is key to complete understanding of molecular mechanisms governing spermatogenesis and the development of new strategies for infertility therapies and male contraception. Here we develop an approach to purify all types of homogeneous spermatogenic cells by combining transgenic labeling and synchronization of the cycle of the seminiferous epithelium, and subsequent single-cell RNA-sequencing. We reveal extensive and previously uncharacterized dynamic processes and molecular signatures in gene expression, as well as specific patterns of alternative splicing, and novel regulators for specific stages of male germ cell development. Our transcriptomics analyses led us to discover discriminative markers for isolating round spermatids at specific stages, and different embryo developmental potentials between early and late stage spermatids, providing evidence that maturation of round spermatids impacts on embryo development. This work provides valuable insights into mammalian spermatogenesis, and a comprehensive resource for future studies towards the complete elucidation of gametogenesis.
Metrics
15 Record Views
Details
- Title
- Single-cell RNA-seq uncovers dynamic processes and critical regulators in mouse spermatogenesis
- Creators
- Yao Chen - State Key Laboratory of Molecular Biology, Shanghai Key Laboratory of Molecular Andrology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai, 200031, ChinaYuxuan Zheng - Peking-Tsinghua Center for Life Sciences, Peking University, Beijing, 100871, ChinaYun Gao - Peking-Tsinghua Center for Life Sciences, Peking University, Beijing, 100871, ChinaZhen Lin - State Key Laboratory of Molecular Biology, Shanghai Key Laboratory of Molecular Andrology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai, 200031, ChinaSuming Yang - State Key Laboratory of Molecular Biology, Shanghai Key Laboratory of Molecular Andrology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai, 200031, ChinaTongtong Wang - State Key Laboratory of Molecular Biology, Shanghai Key Laboratory of Molecular Andrology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai, 200031, ChinaQiu Wang - State Key Laboratory of Molecular Biology, Shanghai Key Laboratory of Molecular Andrology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai, 200031, ChinaNannan Xie - State Key Laboratory of Molecular Biology, Shanghai Key Laboratory of Molecular Andrology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai, 200031, ChinaRong Hua - State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, Jiangsu, 211166, ChinaMingxi Liu - State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, Jiangsu, 211166, ChinaJiahao Sha - State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, Jiangsu, 211166, ChinaMichael D Griswold - School of Molecular Biosciences and the Center for Reproductive Biology, Washington State University, Pullman, WA, USAJinsong Li - State Key Laboratory of Molecular Biology, Shanghai Key Laboratory of Molecular Andrology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai, 200031, China. jsli@sibcb.ac.cnFuchou Tang - Peking-Tsinghua Center for Life Sciences, Peking University, Beijing, 100871, China. tangfuchou@pku.edu.cnMing-Han Tong - State Key Laboratory of Molecular Biology, Shanghai Key Laboratory of Molecular Andrology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai, 200031, China. minghan@sibcb.ac.cn
- Publication Details
- Cell research, Vol.28(9), pp.879-896
- Academic Unit
- Molecular Biosciences, School of
- Publisher
- England
- Identifiers
- 99900547713601842
- Language
- English
- Resource Type
- Journal article