Journal article
Single nucleotide mutation in the barley acetohydroxy acid synthase (AHAS) gene confers resistance to imidazolinone herbicides
Proceedings of the National Academy of Sciences - PNAS, Vol.108(21), pp.8909-8913
05/24/2011
Handle:
https://hdl.handle.net/2376/104283
PMCID: PMC3102390
PMID: 21551103
Abstract
Induced mutagenesis can be an effective way to increase variability in self-pollinated crops for a wide variety of agronomically important traits. Crop resistance to a given herbicide can be of practical value to control weeds with efficient chemical use. In some crops (for example, wheat, maize, and canola), resistance to imidazolinone herbicides (IMIs) has been introduced through mutation breeding and is extensively used commercially. However, this production system imposes plant-back restrictions on rotational crops because of herbicide residuals in the soil. In the case of barley, a preferred rotational crop after wheat, a period of 9–18 mo is required. Thus, introduction of barley varieties showing resistance to IMIs will provide greater flexibility as a rotational crop. The objective of the research reported was to identify resistance in barley for IMIs through induced mutagenesis. To achieve this objective, a sodium azide-treated M
2
/M
3
population of barley cultivar Bob was screened for resistance against acetohydroxy acid synthase (AHAS)-inhibiting herbicides. The phenotypic screening allowed identification of a mutant line showing resistance against IMIs. Molecular analysis identified a single-point mutation leading to a serine 653 to asparagine amino acid substitution in the herbicide-binding site of the barley
AHAS
gene. The transcription pattern of the
AHAS
gene in the mutant (Ser653Asn) and WT has been analyzed, and greater than fourfold difference in transcript abundance was observed. Phenotypic characteristics of the mutant line are promising and provide the base for the release of IMI-resistant barley cultivar(s).
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Details
- Title
- Single nucleotide mutation in the barley acetohydroxy acid synthase (AHAS) gene confers resistance to imidazolinone herbicides
- Creators
- Hyejin Lee - Department of Crop and Soil Sciences andSachin Rustgi - Department of Crop and Soil Sciences andNeeraj Kumar - Department of Crop and Soil Sciences andIan Burke - Department of Crop and Soil Sciences andJoseph P Yenish - Department of Crop and Soil Sciences andKulvinder S Gill - Department of Crop and Soil Sciences andDiter von Wettstein - Department of Crop and Soil Sciences andSteven E Ullrich - Department of Crop and Soil Sciences and
- Publication Details
- Proceedings of the National Academy of Sciences - PNAS, Vol.108(21), pp.8909-8913
- Academic Unit
- Crop and Soil Sciences, Department of
- Publisher
- National Academy of Sciences
- Identifiers
- 99900546742901842
- Language
- English
- Resource Type
- Journal article