Single walled carbon nanotube-based junction biosensor for detection of Escherichia coli

Kara Yamada; Chong-Tai Kim; Jong-Hoon Kim; Jae-Hyun Chung; Hyeon Gyu Lee; Soojin Jun

doi:10.1371/journal.pone.0105767

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Single walled carbon nanotube-based junction biosensor for detection of Escherichia coli

Journal article

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Single walled carbon nanotube-based junction biosensor for detection of Escherichia coli

Kara Yamada, Chong-Tai Kim, Jong-Hoon Kim, Jae-Hyun Chung, Hyeon Gyu Lee and Soojin Jun

PloS one, Vol.9(9), pp.e105767-e105767

2014

DOI: https://doi.org/10.1371/journal.pone.0105767

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https://hdl.handle.net/2376/110665

PMCID: PMC4169404

PMID: 25233366

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Abstract

Foodborne Diseases - microbiology

Gold

Escherichia coli Infections - diagnosis

Antibodies, Immobilized - immunology

Nanotubes, Carbon - microbiology

Food Safety

Electric Conductivity

Foodborne Diseases - diagnosis

Escherichia coli K12 - immunology

Hazard Analysis and Critical Control Points - methods

Sensitivity and Specificity

Tungsten

Antibodies, Bacterial - immunology

Biosensing Techniques - instrumentation

Biosensing Techniques - methods

Foodborne pathogen detection using biomolecules and nanomaterials may lead to platforms for rapid and simple electronic biosensing. Integration of single walled carbon nanotubes (SWCNTs) and immobilized antibodies into a disposable bio-nano combinatorial junction sensor was fabricated for detection of Escherichia coli K-12. Gold tungsten wires (50 µm diameter) coated with polyethylenimine (PEI) and SWCNTs were aligned to form a crossbar junction, which was functionalized with streptavidin and biotinylated antibodies to allow for enhanced specificity towards targeted microbes. In this study, changes in electrical current (ΔI) after bioaffinity reactions between bacterial cells (E. coli K-12) and antibodies on the SWCNT surface were monitored to evaluate the sensor's performance. The averaged ΔI increased from 33.13 nA to 290.9 nA with the presence of SWCNTs in a 10(8) CFU/mL concentration of E. coli, thus showing an improvement in sensing magnitude. Electrical current measurements demonstrated a linear relationship (R2 = 0.973) between the changes in current and concentrations of bacterial suspension in range of 10(2)-10(5) CFU/mL. Current decreased as cell concentrations increased, due to increased bacterial resistance on the bio-nano modified surface. The detection limit of the developed sensor was 10(2) CFU/mL with a detection time of less than 5 min with nanotubes. Therefore, the fabricated disposable junction biosensor with a functionalized SWCNT platform shows potential for high-performance biosensing and application as a detection device for foodborne pathogens.

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Title: Single walled carbon nanotube-based junction biosensor for detection of Escherichia coli
Creators: Kara Yamada - Department of Human Nutrition, Food, and Animal Sciences, University of Hawaii, Honolulu, Hawaii, United States of America
Chong-Tai Kim - Korea Food Research Institute, Seongnam-si, Republic of Korea
Jong-Hoon Kim - Department of Mechanical Engineering, University of Washington, Seattle, Washington, United States of America
Jae-Hyun Chung - Department of Mechanical Engineering, University of Washington, Seattle, Washington, United States of America
Hyeon Gyu Lee - Department of Food and Nutrition, Hanyang University, Seoul, Republic of Korea
Soojin Jun - Department of Human Nutrition, Food, and Animal Sciences, University of Hawaii, Honolulu, Hawaii, United States of America
Publication Details: PloS one, Vol.9(9), pp.e105767-e105767
Academic Unit: Engineering and Computer Science (VANC), School of
Publisher: United States
Identifiers: 99900547554001842
Language: English
Resource Type: Journal article