Journal article
Site-specific DNA repair at the nucleosome level in a yeast minichromosome
Cell (Cambridge), Vol.61(4), pp.675-684
1990
Handle:
https://hdl.handle.net/2376/110383
PMID: 2188732
Abstract
The rate of excision repair of UV-induced pyrimidine dimers (PDs) was measured at specific sites in each strand of a yeast minichromosome containing an active gene (
URA3), a replication origin (
ARS1), and positioned nucleosomes. All six PD sites analyzed in the transcribed
URA3 strand were repaired more rapidly (>5-fold on average) than any of the nine PD sites analyzed in the nontranscribed strand. Efficient repair also occurred in both strands of a disrupted
TRP1 gene (ten PD sites), containing four unstable nucleosomes, and in a nucleosome gap at the 5′ end of
URA3 (two PD sites). Conversely, slow repair occurred in both strands immediately downstream of the
URA3 gene (12 of 14 PD sites). This region contains the
ARS1 consensus sequence, a nucleosome gap, and two stable nucleosomes. Thus, modulation of DNA repair occurs in a simple yeast minichromosome and correlates with gene expression, nucleosome stability, and (possibly) control of replication.
Metrics
15 Record Views
Details
- Title
- Site-specific DNA repair at the nucleosome level in a yeast minichromosome
- Creators
- Michael J Smerdon - Biochemistry/Biophysics Program Washington State University Pullman, Washington 99164-4660 USAFritz Thoma - Institut für Zellbiologie ETH-Hönggerberg CH-8093 Zurich, Switzerland
- Publication Details
- Cell (Cambridge), Vol.61(4), pp.675-684
- Academic Unit
- Molecular Biosciences, School of
- Publisher
- Elsevier Inc
- Identifiers
- 99900547174401842
- Language
- English
- Resource Type
- Journal article