Journal article
Sleep Deprivation Effects on Circadian Clock Gene Expression in the Cerebral Cortex Parallel Electroencephalographic Differences among Mouse Strains
The Journal of neuroscience, Vol.28(28), pp.7193-7201
07/09/2008
Handle:
https://hdl.handle.net/2376/110680
PMCID: PMC2603080
PMID: 18614689
Abstract
Sleep deprivation (SD) results in increased electroencephalographic (EEG) delta power during subsequent non-rapid eye movement sleep (NREMS) and is associated with changes in the expression of circadian clock-related genes in the cerebral cortex. The increase of NREMS delta power as a function of previous wake duration varies among inbred mouse strains. We sought to determine whether SD-dependent changes in circadian clock gene expression parallel this strain difference described previously at the EEG level. The effects of enforced wakefulness of incremental durations of up to 6 h on the expression of circadian clock genes (
bmal1
,
clock
,
cry1
,
cry2
,
csnk1
ε,
npas2
,
per1
, and
per2
) were assessed in AKR/J, C57BL/6J, and DBA/2J mice, three strains that exhibit distinct EEG responses to SD. Cortical expression of clock genes subsequent to SD was proportional to the increase in delta power that occurs in inbred strains: the strain that exhibits the most robust EEG response to SD (AKR/J) exhibited dramatic increases in expression of
bmal1
,
clock
,
cry2
,
csnkI
ε, and
npas2
, whereas the strain with the least robust response to SD (DBA/2) exhibited either no change or a decrease in expression of these genes and
cry1
. The effect of SD on circadian clock gene expression was maintained in mice in which both of the
cryptochrome
genes were genetically inactivated.
cry1
and
cry2
appear to be redundant in sleep regulation as elimination of either of these genes did not result in a significant deficit in sleep homeostasis. These data demonstrate transcriptional regulatory correlates to previously described strain differences at the EEG level and raise the possibility that genetic differences underlying circadian clock gene expression may drive the EEG differences among these strains.
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Details
- Title
- Sleep Deprivation Effects on Circadian Clock Gene Expression in the Cerebral Cortex Parallel Electroencephalographic Differences among Mouse Strains
- Creators
- Jonathan P Wisor - Biosciences Division, SRI International, Menlo Park, California 94025Ravi K Pasumarthi - Biosciences Division, SRI International, Menlo Park, California 94025Dmitry Gerashchenko - Biosciences Division, SRI International, Menlo Park, California 94025Carol L Thompson - Allen Institute for Brain Science, Seattle, Washington 98103Sayan Pathak - Allen Institute for Brain Science, Seattle, Washington 98103Aziz Sancar - Department of Biochemistry and Biophysics, University of North Carolina, Chapel Hill, North Carolina 27599, andPaul Franken - Department of Biological Sciences, Stanford University, Stanford, California 94305Ed S Lein - Allen Institute for Brain Science, Seattle, Washington 98103Thomas S Kilduff - Biosciences Division, SRI International, Menlo Park, California 94025
- Publication Details
- The Journal of neuroscience, Vol.28(28), pp.7193-7201
- Academic Unit
- Biomedical Sciences, Department of
- Publisher
- Society for Neuroscience
- Identifiers
- 99900547393201842
- Language
- English
- Resource Type
- Journal article