Journal article
Sleep deprivation increases the activation of nuclear factor kappa B in lateral hypothalamic cells
Brain research, Vol.1004(1), pp.91-97
2004
Handle:
https://hdl.handle.net/2376/106390
PMID: 15033423
Abstract
Sleep deprivation increases sleep propensity in rats and mice as well as the production of several sleep-regulatory substances. Nuclear factor kappa B (NF-κB) is a transcription factor implicated in the activation of many of these sleep-promoting substances. A unique population of neurons immunoreactive for the p65 subunit of NF-κB was previously localized within the caudal dorsolateral hypothalamus of rats. Therefore, we evaluated the effect of sleep deprivation on NF-κBp65-immunoreactivity (IR) in cells of this region in rats as well as its nuclear translocation in a κB-
lacZ transgenic mouse line. In rats after 6 h of sleep deprivation beginning at light onset, the number of neurons with NF-κBp65-IR increased significantly in the caudal lateral hypothalamus, specifically the magnocellular lateral hypothalamus adjacent to the subthalamus. Sleep deprivation also significantly increased the number of cells expressing NF-κB-dependent β-galactosidase in the magnocellular lateral hypothalamus, zona incerta dorsal, as well as the adjacent subthalamus in the transgenic mice. These results suggest that NF-κB expressing cells within the lateral hypothalamus may be important in the maintenance of the sleep–wake cycle.
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Details
- Title
- Sleep deprivation increases the activation of nuclear factor kappa B in lateral hypothalamic cells
- Creators
- Judith A Brandt - Department of Veterinary and Comparative Anatomy, Pharmacology and Physiology, College of Veterinary Medicine, Washington State University, P.O. Box 646520, Pullman, WA 99164-6520, USALynn Churchill - Department of Veterinary and Comparative Anatomy, Pharmacology and Physiology, College of Veterinary Medicine, Washington State University, P.O. Box 646520, Pullman, WA 99164-6520, USAAbdur Rehman - Department of Veterinary and Comparative Anatomy, Pharmacology and Physiology, College of Veterinary Medicine, Washington State University, P.O. Box 646520, Pullman, WA 99164-6520, USAGeorgeann Ellis - Department of Veterinary and Comparative Anatomy, Pharmacology and Physiology, College of Veterinary Medicine, Washington State University, P.O. Box 646520, Pullman, WA 99164-6520, USASylvie Mémet - Unité de Biologie Moléculaire de l'Expression Génique, URA CNRS 2582, Institut Pasteur, 28 rue du Dr Roux, 75724 Paris Cedex 15, FranceAlain Israël - Unité de Biologie Moléculaire de l'Expression Génique, URA CNRS 2582, Institut Pasteur, 28 rue du Dr Roux, 75724 Paris Cedex 15, FranceJames M Krueger - Department of Veterinary and Comparative Anatomy, Pharmacology and Physiology, College of Veterinary Medicine, Washington State University, P.O. Box 646520, Pullman, WA 99164-6520, USA
- Publication Details
- Brain research, Vol.1004(1), pp.91-97
- Academic Unit
- Integrative Physiology and Neuroscience, Department of
- Publisher
- Elsevier B.V
- Identifiers
- 99900546518801842
- Language
- English
- Resource Type
- Journal article