Journal article
Sleep in mice with nonfunctional growth hormone-releasing hormone receptors
American journal of physiology. Regulatory, integrative and comparative physiology, Vol.284(1), pp.R131-R139
01/01/2003
Handle:
https://hdl.handle.net/2376/108350
PMID: 12388430
Abstract
The role of the somatotropic axis in sleep regulation was studied by using the lit/lit mouse with nonfunctional growth hormone (GH)-releasing hormone (GHRH) receptors (GHRH-Rs) and control heterozygous C57BL/6J mice, which have a normal phenotype. During the light period, the lit/lit mice displayed significantly less spontaneous rapid eye movement sleep (REMS) and non-REMS (NREMS) than the controls. Intraperitoneal injection of GHRH (50 μg/kg) failed to promote sleep in the lit/lit mice, whereas it enhanced NREMS in the heterozygous mice. Subcutaneous infusion of GH replacement stimulated weight gain, increased the concentration of plasma insulin-like growth factor-1 (IGF-1), and normalized REMS, but failed to restore normal NREMS in the lit/lit mice. The NREMS response to a 4-h sleep deprivation was attenuated in the lit/lit mice. In control mice, intraperitoneal injection of ghrelin (400 μg/kg) elicited GH secretion and promoted NREMS, and intraperitoneal administration of the somatostatin analog octretotide (Oct, 200 μg/kg) inhibited sleep. In contrast, these responses were missing in the lit/lit mice. The results suggest that GH promotes REMS whereas GHRH stimulates NREMS via central GHRH-Rs and that GHRH is involved in the mediation of the sleep effects of ghrelin and somatostatin.
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Details
- Title
- Sleep in mice with nonfunctional growth hormone-releasing hormone receptors
- Creators
- Ferenc Obal - Department of Physiology and, Department of Veterinary and Comparative Anatomy, Pharmacology and Physiology, Washington State University, Pullman, Washington 99164-6520Jeremiah Alt - Department of Veterinary and Comparative Anatomy, Pharmacology and Physiology, Washington State University, Pullman, Washington 99164-6520Ping Taishi - Department of Veterinary and Comparative Anatomy, Pharmacology and Physiology, Washington State University, Pullman, Washington 99164-6520Janos Gardi - Endocrine Unit, University of Szeged, A. Szent-Györgyi Medical Center, 6720 Szeged, Hungary; andJames M Krueger - Department of Veterinary and Comparative Anatomy, Pharmacology and Physiology, Washington State University, Pullman, Washington 99164-6520
- Publication Details
- American journal of physiology. Regulatory, integrative and comparative physiology, Vol.284(1), pp.R131-R139
- Academic Unit
- Integrative Physiology and Neuroscience, Department of
- Identifiers
- 99900547043301842
- Language
- English
- Resource Type
- Journal article