Journal article
Small heat shock protein HSPB1 regulates growth of embryonic zebrafish craniofacial muscles
Experimental cell research, Vol.319(6), pp.860-874
04/01/2013
Handle:
https://hdl.handle.net/2376/111883
PMID: 23313812
Abstract
The small heat shock protein HspB1 (Hsp27) is abundantly expressed in embryonic muscle tissues of a wide variety of vertebrate species. However, the functional significance of this expression pattern is not well established. In the present study, we observed specific, high level expression of HspB1 protein and an HspB1 gene reporter in developing craniofacial muscles of the zebrafish, Danio rerio, and examined the consequences of reducing HspB1 expression to the development and growth of these muscles. Quantitative morphometric analyses revealed a reduction in the cross-sectional area of myofibers in embryos expressing reduced HspB1 levels by as much as 47% compared to controls. In contrast, we detected no differences in the number of myofibrils or associated nuclei, nor the number, size or development of chondrocytes in surrounding tissues. We also did not detect changes to the overall organization of sarcomeres or myofibrils in embryos expressing reduced levels of HspB1. Together our results reveal a critical role for HspB1 in the growth of myofibrils and provide new insight into the mechanism underlying its developmental function.
► High HspB1 expression levels characterize craniofacial muscles in zebrafish embryos. ► HspB1 does not associate with myofibrils during normal development. ► Morpholino injection was used to reduce HspB1 expression in zebrafish embryos. ► Loss of HspB1 specifically reduces myofibril size in embryonic craniofacial muscles. ► Loss of HspB1 does not affect embryonic craniofacial tissues lacking HspB1.
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Details
- Title
- Small heat shock protein HSPB1 regulates growth of embryonic zebrafish craniofacial muscles
- Creators
- Ryan C Middleton - School of Molecular Biosciences, College of Veterinary Medicine, Washington State University, Pullman, WA, USAEric A Shelden - School of Molecular Biosciences, College of Veterinary Medicine, Washington State University, Pullman, WA, USA
- Publication Details
- Experimental cell research, Vol.319(6), pp.860-874
- Academic Unit
- Molecular Biosciences, School of
- Publisher
- Elsevier Inc
- Identifiers
- 99900547597301842
- Language
- English
- Resource Type
- Journal article