Journal article
Smart Drug Delivery System-Inspired Enzyme-Linked Immunosorbent Assay Based on Fluorescence Resonance Energy Transfer and Allochroic Effect Induced Dual-Modal Colorimetric and Fluorescent Detection
Analytical chemistry (Washington), Vol.90(3), pp.1976-1982
02/06/2018
PMID: 29307181
Abstract
Numerous analytical techniques have been undertaken for the detection of protein biomarkers because of their extensive and significant applications in clinical diagnosis, whereas there are few strategies to develop dual-readout immunosensors to achieve more accurate results. To the best of our knowledge, inspired by smart drug delivery system (DDS), a novel pH-responsive modified enzyme-linked immunosorbent assay (ELISA) was innovatively developed for the first time, realizing dual-modal colorimetric and fluorescent detection of cardiac troponin I (cTnI). Curcumin (CUR) was elaborately selected as a reporter molecule, which played the same role of drugs in DDS based on the following considerations: (1) CUR can be used as a kind of pH indicator by the inherited allochroic effect induced by basic pH value; (2) the fluorescence of CUR can be quenched by certain nanocarriers as the acceptor because of the occurrence of fluorescence resonance energy transfer (FRET), while recovered by the stimuli of basic pH value, which can produce "signal-on" fluorescence detection. Three-dimensional MoS2 nanoflowers (3D-MoS2 NFs) were employed in immobilizing CUR to constitute a nanoprobe for the determination of cTnI by virtue of good biocompatibility, high absorption capacity, and fluorescence quench efficiency toward CUR The proposed DDS-inspired ELISA offered dual-modal colorimetric and fluorescent detection of cTnI, thereby meeting the reliable and precise analysis requirements. We believe that the developed dual-readout ELISA will create a new avenue and bring innovative inspirations for biological detections.
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Details
- Title
- Smart Drug Delivery System-Inspired Enzyme-Linked Immunosorbent Assay Based on Fluorescence Resonance Energy Transfer and Allochroic Effect Induced Dual-Modal Colorimetric and Fluorescent Detection
- Creators
- Luyang Miao - University of JinanChengzhou Zhu - Washington State UniversityLei Jiao - Chengdu University of Information TechnologyHe Li - Chengdu University of Information TechnologyDan Du - Washington State UniversityYuehe Lin - Washington State UniversityQin Wei - University of Jinan
- Publication Details
- Analytical chemistry (Washington), Vol.90(3), pp.1976-1982
- Academic Unit
- School of Mechanical and Materials Engineering
- Publisher
- Amer Chemical Soc
- Number of pages
- 7
- Grant note
- ZR2017MB017 / Natural Science Foundation of Shandong; Natural Science Foundation of Shandong Province 21245007; 81000976 / Natural Science Foundation of China; National Natural Science Foundation of China (NSFC)
- Identifiers
- 99901227637201842
- Language
- English
- Resource Type
- Journal article