Journal article
Smartphone-Based Dual-Channel Immunochromatographic Test Strip with Polymer Quantum Dot Labels for Simultaneous Detection of Cypermethrin and 3-Phenoxybenzoic Acid
Analytical chemistry (Washington), Vol.93(40), pp.13658-13666
10/12/2021
PMID: 34591463
Abstract
Currently, gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-MS (LC-MS) are the primary methods used to detect pesticides and their metabolites for biomonitoring of exposure. Although GC-MS and LC-MS can provide accurate and sensitive measurements, these techniques are not suitable for point-of-care or in-field biomonitoring applications. The objective of this work is to develop a smartphone-based dual-channel immunochromatographic test strip (ICTS) for on-site biomonitoring of exposure to cypermethrin by simultaneous detection of cypermethrin and its metabolite, 3-phenoxybenzoic acid (3-PBA). Polymer carbon dots (PCDs) with ultrahigh fluorescent brightness were synthesized and used as a signal amplifier in ICTS assay. Cypermethrin (a representative pyrethroid pesticide) and its major metabolite 3-PBA were simultaneously detected to provide more comprehensive analysis of cypermethrin exposure. After competitive immunoreactions between the target sample and the coating antigens preloaded on the test line, the tracer antibody (PCD-conjugated antibody) was quantitatively captured on the test lines. The captured PCDs were inversely proportional to the amount of the target compound in the sample. The red fluorescence on the test line was then recorded using a smartphone-based device capable of conducting image analysis and recording. Under optimal conditions, the sensor showed excellent linear responses for detecting cypermethrin and 3-PBA ranging from 1 to 100 ng/mL and from 0.1 to 100 ng/mL, respectively, and the limits of detection were calculated to be similar to 0.35 ng/mL for cypermethrin and similar to 0.04 ng/mL for 3-PBA. The results demonstrate that the ICTS device is promising for accurate point-of-care biomonitoring of pesticide exposure.
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Details
- Title
- Smartphone-Based Dual-Channel Immunochromatographic Test Strip with Polymer Quantum Dot Labels for Simultaneous Detection of Cypermethrin and 3-Phenoxybenzoic Acid
- Creators
- Yuting Zhao - Washington State UniversityXiaofan Ruan - Washington State UniversityYang Song - Washington State UniversityJordan N. Smith - Pacific Northwest National LaboratoryNatalia Vasylieva - University of California, DavisBruce D. Hammock - University of California, DavisYuehe Lin - Washington State UniversityDan Du - Washington State University
- Publication Details
- Analytical chemistry (Washington), Vol.93(40), pp.13658-13666
- Academic Unit
- School of Mechanical and Materials Engineering
- Publisher
- Amer Chemical Soc
- Number of pages
- 9
- Grant note
- R35 ES030443-01 / National Institute of Environmental Health Sciences RIVER Award (NIEHS) R01OH011023-01A1 / Centers for Disease Control and Prevention/National Institute for Occupational Safety and Health (CDC/NIOSH); United States Department of Health & Human Services; Centers for Disease Control & Prevention - USA; National Institute for Occupational Safety & Health (NIOSH) P42ES004699 / National Institute of Environmental Health Science Superfund Research Program
- Identifiers
- 99901227638801842
- Language
- English
- Resource Type
- Journal article