Journal article
Spatial Patterns of DNA Replication, Protein Synthesis, and Oxygen Concentration within Bacterial Biofilms Reveal Diverse Physiological States
Journal of bacteriology, Vol.189(11), pp.4223-4233
06/2007
Handle:
https://hdl.handle.net/2376/114937
PMCID: PMC1913414
PMID: 17337582
Abstract
It has long been suspected that microbial biofilms harbor cells in a variety of activity states, but there have been few direct experimental visualizations of this physiological heterogeneity. Spatial patterns of DNA replication and protein synthetic activity were imaged and quantified in staphylococcal biofilms using immunofluorescent detection of pulse-labeled DNA and also an inducible green fluorescent protein (GFP) construct. Stratified patterns of DNA synthetic and protein synthetic activity were observed in all three biofilm systems to which the techniques were applied. In a colony biofilm system, the dimensions of the zone of anabolism at the air interface ranged from 16 to 38 μm and corresponded with the depth of oxygen penetration measured with a microelectrode. A second zone of activity was observed along the nutrient interface of the biofilm. Much of the biofilm was anabolically inactive. Since dead cells constituted only 10% of the biofilm population, most of the inactive cells in the biofilm were still viable. Collectively, these results suggest that staphylococcal biofilms contain cells in at least four distinct states: growing aerobically, growing fermentatively, dead, and dormant. The variety of activity states represented in a biofilm may contribute to the special ecology and tolerance to antimicrobial agents of biofilms.
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Details
- Title
- Spatial Patterns of DNA Replication, Protein Synthesis, and Oxygen Concentration within Bacterial Biofilms Reveal Diverse Physiological States
- Creators
- Suriani Abdul Rani - Center for Biofilm EngineeringBetsey Pitts - Center for Biofilm EngineeringHaluk Beyenal - Center for Biofilm EngineeringRaaja Angathevar Veluchamy - Center for Biofilm EngineeringZbigniew Lewandowski - Center for Biofilm EngineeringWilliam M Davison - Center for Biofilm EngineeringKelli Buckingham-Meyer - Center for Biofilm EngineeringPhilip S Stewart - Center for Biofilm Engineering
- Publication Details
- Journal of bacteriology, Vol.189(11), pp.4223-4233
- Academic Unit
- Chemical Engineering and Bioengineering, School of
- Publisher
- American Society for Microbiology
- Identifiers
- 99900547554601842
- Language
- English
- Resource Type
- Journal article