Journal article
Spatial and temporal regulation of the forisome gene for1 in the phloem during plant development
Plant molecular biology, Vol.65(3), pp.285-294
10/2007
Handle:
https://hdl.handle.net/2376/117674
PMID: 17694275
Abstract
Forisomes are protein aggregates found uniquely in the sieve elements of Fabaceaen plants. Upon wounding they undergo a reversible, calcium-dependent conformational switch which enables them to act as cellular stopcocks. Forisomes begin to form in young sieve elements at an early stage of metaphloem differentiation. Genes encoding forisome components could therefore be useful as markers of early sieve element development. Here we present a comprehensive analysis of the developmental expression profile of for1, which encodes such a forisome component. The for1 gene is highly conserved among Fabaceaen species and appears to be unique to this phylogenetic lineage since no orthologous genes have been found in other plants, including Arabidopsis and rice. Even so, transgenic tobacco plants expressing reporter genes under the control of the for1 promoter display reporter activity exclusively in immature sieve elements. This suggests that the regulation of sieve element development is highly conserved even in plants where mature forisomes have not been detected. The promoter system could therefore provide a powerful tool for the detailed analysis of differentiation in metaphloem sieve elements in an unexpectedly broad range of plant species.
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Details
- Title
- Spatial and temporal regulation of the forisome gene for1 in the phloem during plant development
- Creators
- Gundula Noll - Institut für Allgemeine Botanik der Justus Liebig Universität Gießen Senckenbergstr. 17-21 35390 Giessen GermanyMaria Fontanellaz - Fraunhofer Institut für Molekularbiologie und Angewandte Ökologie, Bereich Molekularbiologie Auf dem Aberg 1 57392 Schmallenberg GermanyBoris Rüping - Institut für Biochemie und Biotechnologie der Pflanzen der Westfälischen Wilhelms-Universität Münster Hindenburgplatz 55 48143 Munster GermanyAhmed Ashoub - Agricultural Genetic Engineering Research Institute Agricultural Research Centre 9 Gamaa St 12619 Giza EgyptAart van Bel - Institut für Allgemeine Botanik der Justus Liebig Universität Gießen Senckenbergstr. 17-21 35390 Giessen GermanyRainer Fischer - Fraunhofer Institut für Molekularbiologie und Angewandte Ökologie, Bereich Molekularbiologie Auf dem Aberg 1 57392 Schmallenberg GermanyMichael Knoblauch - School of Biological Sciences Washington State University Pullmann WA 99164-4236 USADirk Prüfer - Fraunhofer Institut für Molekularbiologie und Angewandte Ökologie, Bereich Molekularbiologie Auf dem Aberg 1 57392 Schmallenberg Germany
- Publication Details
- Plant molecular biology, Vol.65(3), pp.285-294
- Academic Unit
- Biological Sciences, School of
- Publisher
- Springer Netherlands; Dordrecht
- Identifiers
- 99900547828501842
- Language
- English
- Resource Type
- Journal article