Journal article
Specific chromosome 1 breaks induced by human cytomegalovirus
Proceedings of the National Academy of Sciences - PNAS, Vol.97(2), pp.853-858
01/18/2000
Handle:
https://hdl.handle.net/2376/106003
PMCID: PMC15420
PMID: 10639169
Abstract
Human cytomegalovirus (HCMV) is the major viral cause of birth defects and a serious problem for immunocompromised individuals. Here we show that infection of cells with HCMV during the S-phase of the cell cycle results in two specific chromosome 1 breaks at positions 1q42 and 1q21. We demonstrate that purified virions, and not infected cell supernatant alone, are responsible for the damage. In addition, we show that the specific breaks occur when different sources of fibroblasts and strains of HCMV are used. Incubation of the virus with neutralizing antibody prevents the induction of breaks. However, UV-inactivated virus is as efficient as untreated virus in inducing specific damage to chromosome 1. Thus, there is a requirement for viral adsorption/penetration, but not new viral gene expression. This HCMV-mediated induction of site-specific damage in actively dividing cells may provide clues for the development of neurological defects in the congenitally infected infant.
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Details
- Title
- Specific chromosome 1 breaks induced by human cytomegalovirus
- Creators
- Elizabeth A Fortunato - Department of Biology, Center for Molecular Genetics andMarie L Dell'Aquila - Department of Biology, Center for Molecular Genetics andDeborah H Spector - Department of Biology, Center for Molecular Genetics and
- Publication Details
- Proceedings of the National Academy of Sciences - PNAS, Vol.97(2), pp.853-858
- Academic Unit
- UNKNOWN
- Publisher
- The National Academy of Sciences
- Identifiers
- 99900546810401842
- Language
- English
- Resource Type
- Journal article