Journal article
Specific erythrocyte binding is an additional nutrient acquisition system for Trichomonas vaginalis
The Journal of experimental medicine, Vol.171(6), pp.2165-2170
06/01/1990
Handle:
https://hdl.handle.net/2376/104853
PMCID: PMC2187958
PMID: 2351937
Abstract
Specific receptor-mediated binding by Trichomonas vaginalis of human erythrocytes was demonstrated. The ability of live parasites to internalize erythrocytes was also documented. In vitro growth assays during lipid-free and iron-limiting conditions that do not support the survival of T. vaginalis organisms showed that purified erythrocyte lipids and hemoglobin were each able to provide lipids and/or hemoglobin iron for trichomonal growth and multiplication. Parasites bound hemoglobin in a highly specific receptor-mediated fashion, and only the homologous unlabeled hemoglobin, but not lactoferrin and transferrin, competed with iodinated hemoglobin binding. Two antibody-crossreactive surface proteins of the parasites were identified as adhesins, and antibody to the individual adhesins inhibited T. vaginalis recognition and binding of erythrocytes. Finally, patient sera possessed antibody to the adhesins, showing the immunogenic nature and in vivo relevance of the trichomonad proteins during infection.
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Details
- Title
- Specific erythrocyte binding is an additional nutrient acquisition system for Trichomonas vaginalis
- Creators
- M W Lehker - Department of Microbiology, University of Texas Health Science Center, San Antonio 78284T H ChangD C DaileyJ F Alderete
- Publication Details
- The Journal of experimental medicine, Vol.171(6), pp.2165-2170
- Academic Unit
- Molecular Biosciences, School of
- Publisher
- United States
- Grant note
- AI-22380 / NIAID NIH HHS T32-AI-07271 / NIAID NIH HHS
- Identifiers
- 99900546803401842
- Language
- English
- Resource Type
- Journal article