Journal article
Specific parasitism of purified vaginal epithelial cells by Trichomonas vaginalis
Infection and immunity, Vol.56(10), pp.2558-2562
10/1988
Handle:
https://hdl.handle.net/2376/110562
PMCID: PMC259611
PMID: 3262088
Abstract
Human vaginal epithelial cells (VECs) from vaginal swabs obtained from normal women or from patients with trichomoniasis were purified, and VEC parasitism by Trichomonas vaginalis was examined. Trichomonads bound equally well to live or dead VECs, and up to 20% of VECs were parasitized. Trichomonal cytadherence of human VECs was time, temperature, and pH dependent. Saturation binding levels of live trichomonads to VECs gave approximately 2 organisms adherent to parasitized VEC. No differences in cytadherence levels were detected by different isolates to VECs from the same patient compared with adherence to VECs from normal individuals. Trypsinized, live T. vaginalis organisms failed to recognize VECs. A ligand assay identified four adhesin candidates, and only organisms without a prominent immunogen on the surface (negative phenotype) cytadhered to VECs and synthesized the adhesins, confirming the results of a recently published report by us on adherence to HeLa cell monolayers (J. F. Alderete and G. E. Garza, Infect. Immun. 56:28-33, 1988). These data show the ability of T. vaginalis to parasitize human vaginal epithelial cells in a specific receptor-ligand manner.
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Details
- Title
- Specific parasitism of purified vaginal epithelial cells by Trichomonas vaginalis
- Creators
- J F Alderete - Department of Microbiology, University of Texas Health Science Center, San Antonio 78284P Demeś - Department of Microbiology, University of Texas Health Science Center, San Antonio 78284A Gombosova - Department of Microbiology, University of Texas Health Science Center, San Antonio 78284M Valent - Department of Microbiology, University of Texas Health Science Center, San Antonio 78284M Fabusová - Department of Microbiology, University of Texas Health Science Center, San Antonio 78284A Jánoska - Department of Microbiology, University of Texas Health Science Center, San Antonio 78284J Stefanovic - Department of Microbiology, University of Texas Health Science Center, San Antonio 78284R Arroyo - Department of Microbiology, University of Texas Health Science Center, San Antonio 78284
- Publication Details
- Infection and immunity, Vol.56(10), pp.2558-2562
- Academic Unit
- Molecular Biosciences, School of
- Identifiers
- 99900547237801842
- Language
- English
- Resource Type
- Journal article