Spontaneous and influenza virus-induced sleep are altered in TNF-α double-receptor deficient mice

Levente Kapás; Stewart G Bohnet; Tim R Traynor; Jeannine A Majde; Éva Szentirmai; Paul Magrath; Ping Taishi; James M Krueger

doi:10.1152/japplphysiol.90388.2008

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Spontaneous and influenza virus-induced sleep are altered in TNF-α double-receptor deficient mice

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Spontaneous and influenza virus-induced sleep are altered in TNF-α double-receptor deficient mice

Levente Kapás, Stewart G Bohnet, Tim R Traynor, Jeannine A Majde, Éva Szentirmai, Paul Magrath, Ping Taishi and James M Krueger

Journal of applied physiology (1985), Vol.105(4), pp.1187-1198

10/2008

DOI: https://doi.org/10.1152/japplphysiol.90388.2008

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https://hdl.handle.net/2376/108542

PMCID: PMC2576045

PMID: 18687977

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Abstract

cytokine

orexin

hypothermia

purine receptor

electroencephalogram delta power

Tumor necrosis factor-α (TNF-α) is associated with sleep regulation in health and disease. Previous studies assessed sleep in mice genetically deficient in the TNF-α 55-kDa receptor. In this study, spontaneous and influenza virus-induced sleep profiles were assessed in mice deficient in both the 55-kDa and 75-kDa TNF-α receptors [TNF-2R knockouts (KO)] and wild-type (WT) strain controls. Under baseline conditions the TNF-2R KO mice had less non-rapid eye movement sleep (NREMS) than WTs during the nighttime and more rapid eye movement sleep (REMS) than controls during the daytime. The differences between nighttime maximum and daytime minimum values of electroencephalogram (EEG) delta power during NREMS were greater in the TNF-2R KO mice than in WTs. Viral challenge (mouse-adapted influenza X-31) enhanced NREMS and decreased REMS in both strains roughly to the same extent. EEG delta power responses to viral challenge differed substantially between strains; the WT animals increased, whereas the TNF-2R KO mice decreased their EEG delta wave power during NREMS. There were no differences between strains in body temperatures or locomotor activity in uninfected mice or after viral challenge. Analyses of cortical mRNAs confirmed that the TNF-2R KO mice lacked both TNF-α receptors; these mice also had higher levels of orexin mRNA and reduced levels of the purine P2X7 receptor compared with WTs. Results reinforce the hypothesis that TNF-α is involved in physiological sleep regulation but plays a limited role in the acute-phase response induced by influenza virus.

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Title: Spontaneous and influenza virus-induced sleep are altered in TNF-α double-receptor deficient mice
Creators: Levente Kapás - Department of Biological Sciences, Fordham University, Bronx, New York; and
Stewart G Bohnet - Department of Biological Sciences, Fordham University, Bronx, New York; and
Tim R Traynor - Department of Biological Sciences, Fordham University, Bronx, New York; and
Jeannine A Majde - Department of Biological Sciences, Fordham University, Bronx, New York; and
Éva Szentirmai - Department of Biological Sciences, Fordham University, Bronx, New York; and
Paul Magrath - Department of Biological Sciences, Fordham University, Bronx, New York; and
Ping Taishi - Department of Biological Sciences, Fordham University, Bronx, New York; and
James M Krueger - Department of Biological Sciences, Fordham University, Bronx, New York; and
Publication Details: Journal of applied physiology (1985), Vol.105(4), pp.1187-1198
Academic Unit: Biomedical Sciences, Department of; Integrative Physiology and Neuroscience, Department of
Publisher: American Physiological Society
Identifiers: 99900547003801842
Language: English
Resource Type: Journal article