Stability and Tick Transmission Phenotype of gfp-Transformed Anaplasma marginale through a Complete In Vivo Infection Cycle

Susan M Noh; Massaro W Ueti; Guy H Palmer; Ulrike G Munderloh; Roderick F Felsheim; Kelly A Brayton

doi:10.1128/AEM.02096-10

Back

Stability and Tick Transmission Phenotype of gfp-Transformed Anaplasma marginale through a Complete In Vivo Infection Cycle

Journal article

Open access

Peer reviewed

Stability and Tick Transmission Phenotype of gfp-Transformed Anaplasma marginale through a Complete In Vivo Infection Cycle

Susan M Noh, Massaro W Ueti, Guy H Palmer, Ulrike G Munderloh, Roderick F Felsheim and Kelly A Brayton

Applied and environmental microbiology, Vol.77(1), pp.330-334

01/2011

DOI: https://doi.org/10.1128/AEM.02096-10

Handle:

https://hdl.handle.net/2376/109169

PMCID: PMC3019711

PMID: 21057014

Files and links (1)

url

https://doi.org/10.1128/AEM.02096-10View

Published (Version of record) Open

Abstract

Invertebrate Microbiology

We tested the stability and tick transmission phenotype of transformed Anaplasma marginale through a complete in vivo infection cycle. Similar to the wild type, the gfp -transformed A. marginale strain established infection in cattle, a natural reservoir host, and persisted in immune competent animals. The tick infection rates for the transformed A. marginale and the wild type were the same. However, there were significantly lower levels of the transformed A. marginale than of the wild type in the tick. Despite the lower levels of replication, ticks transmitted the transformant. Transformants can serve as valuable tools to dissect the molecular requirements of tick colonization and pathogen transmission.

Metrics

3 Record Views

Details

Title: Stability and Tick Transmission Phenotype of gfp-Transformed Anaplasma marginale through a Complete In Vivo Infection Cycle
Creators: Susan M Noh - Animal Disease Research Unit, Agricultural Research Service, U.S. Department of Agriculture, Pullman, Washington 99164-7030
Massaro W Ueti - Animal Disease Research Unit, Agricultural Research Service, U.S. Department of Agriculture, Pullman, Washington 99164-7030
Guy H Palmer - Animal Disease Research Unit, Agricultural Research Service, U.S. Department of Agriculture, Pullman, Washington 99164-7030
Ulrike G Munderloh - Animal Disease Research Unit, Agricultural Research Service, U.S. Department of Agriculture, Pullman, Washington 99164-7030
Roderick F Felsheim - Animal Disease Research Unit, Agricultural Research Service, U.S. Department of Agriculture, Pullman, Washington 99164-7030
Kelly A Brayton - Animal Disease Research Unit, Agricultural Research Service, U.S. Department of Agriculture, Pullman, Washington 99164-7030
Publication Details: Applied and environmental microbiology, Vol.77(1), pp.330-334
Academic Unit: Veterinary Microbiology and Pathology, Department of; Paul G. Allen School for Global Animal Health
Publisher: American Society for Microbiology (ASM)
Identifiers: 99900547253701842
Language: English
Resource Type: Journal article