Journal article
Stability of gamma-glutamyltransferase activity in calf sera after refrigerated or frozen storage
American journal of veterinary research, Vol.58(4), pp.354-355
04/1997
Handle:
https://hdl.handle.net/2376/112363
PMID: 9099377
Abstract
To examine stability of -glutamyltransferase (GGT) activity in stored serum from neonatal calves.
10 commercial beef calves between 36 and 60 hours old.
Serum samples were obtained from the calves, and each sample was divided into 8 aliquots. Serum GGT activity was measured on day 0 (fresh) and days 1, 2, 3, and 4 of refrigerated storage (4 C) and weeks 1, 2, and 3 of frozen storage (-20 C).
Serum GGT activities for each of the refrigerated aliquots did not significantly differ from day zero, with serum GGT activity (expressed as a percentage of initial activity) > 99% on all 4 days. Serum GGT activity in frozen aliquots decreased significantly after 1 and 2 weeks of frozen storage, 97 and 98%, respectively; however, this decrease in GGT activity was not biologically significant. The observed GGT activity did not decrease significantly in the samples stored frozen for 3 weeks; these samples retained 99% of initial activity.
The observed stability of serum GGT activity indicates that serum may be obtained, stored, and batch processed at a later time. This stability during storage is important to the success of a bovine passive transfer monitoring program based on GGT activity.
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Details
- Title
- Stability of gamma-glutamyltransferase activity in calf sera after refrigerated or frozen storage
- Creators
- F Muller - Department of Veterinary Clinical Sciences, Washington State University, Pullman 99164, USAJ W TylerS M ParishK A JohnsonD S KrytenbergL K Wilson
- Publication Details
- American journal of veterinary research, Vol.58(4), pp.354-355
- Academic Unit
- Animal Sciences, Department of
- Publisher
- United States
- Identifiers
- 99900547896601842
- Language
- English
- Resource Type
- Journal article