Journal article
Stimulation of Homology-Directed Repair at I-SceI-Induced DNA Breaks during the Permissive Life Cycle of Human Cytomegalovirus
Journal of virology, Vol.85(12), pp.6049-6054
Notes
06/2011
Handle:
https://hdl.handle.net/2376/110096
PMCID: PMC3126324
PMID: 21490102
Abstract
Human cytomegalovirus (HCMV) selectively relocalizes many DNA repair proteins, thereby avoiding a potentially detrimental damage response. In the present study, we evaluated interactions between HCMV and the homology-directed repair (HDR) pathway. In permissive human foreskin fibroblasts, a fluorescence-based double-stranded break repair assay was used to determine that HCMV stimulated HDR. Repair of both stably integrated and extrachromosomal reporter substrates was observed to increase. HDR was also stimulated through individual expression of the viral immediate-early protein IE1-72, mimicking full virus infection. These experiments further demonstrate HCMV's role in modulating critical cellular processes during a permissive infection.
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Details
- Title
- Stimulation of Homology-Directed Repair at I-SceI-Induced DNA Breaks during the Permissive Life Cycle of Human Cytomegalovirus
- Creators
- A. S KulkarniE. A Fortunato
- Publication Details
- Journal of virology, Vol.85(12), pp.6049-6054
- Academic Unit
- UNKNOWN
- Series
- Notes
- Publisher
- American Society for Microbiology; 1752 N St., N.W., Washington, DC
- Identifiers
- 99900547199301842
- Language
- English
- Resource Type
- Journal article