Journal article
Stimulation of Innate Immune Cells by Light-Activated TLR7/8 Agonists
Journal of the American Chemical Society, Vol.136(31), pp.10823-10825
08/06/2014
Handle:
https://hdl.handle.net/2376/114830
PMCID: PMC4132957
PMID: 25029205
Abstract
The innate immune response is controlled, in part, by the synergistic interaction of multiple Toll-like receptors (TLRs). This multi-receptor cooperation is responsible for the potent activity of many vaccines, but few tools have been developed to understand the spatio-temporal elements of TLR synergies. In this Communication , we present photo-controlled agonists of TLR7/8. By strategically protecting the active agonist moiety based on an agonist-bound crystal structure, TLR activity is suppressed and then regained upon exposure to light. We confirmed NF-κB production upon light exposure in a model macrophage cell line. Primary cell activity was confirmed by examining cytokine and cell surface marker production in bone-marrow-derived dendritic cells. Finally, we used light to activate dendritic cell sub-populations within a larger population.
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Details
- Title
- Stimulation of Innate Immune Cells by Light-Activated TLR7/8 Agonists
- Creators
- Keun Ah Ryu - Department of Chemistry, University of California, Irvine, California 92697, United StatesLalisa Stutts - Department of Chemistry, University of California, Irvine, California 92697, United StatesJanine K Tom - Department of Chemistry, University of California, Irvine, California 92697, United StatesRock J Mancini - Department of Chemistry, University of California, Irvine, California 92697, United StatesAaron P Esser-Kahn - Department of Chemistry, University of California, Irvine, California 92697, United States
- Publication Details
- Journal of the American Chemical Society, Vol.136(31), pp.10823-10825
- Academic Unit
- Department of Chemistry
- Number of pages
- 3
- Identifiers
- 99900547694601842
- Language
- English
- Resource Type
- Journal article