Journal article
Stimulation of T-Helper Cell Gamma Interferon and Immunoglobulin G Responses Specific for Babesia bovis Rhoptry-Associated Protein 1 (RAP-1) or a RAP-1 Protein Lacking the Carboxy-Terminal Repeat Region Is Insufficient To Provide Protective Immunity against Virulent B. bovis Challenge
Infection and immunity, Vol.71(9), pp.5021-5032
09/2003
Handle:
https://hdl.handle.net/2376/104749
PMCID: PMC187345
PMID: 12933845
Abstract
Rhoptry-associated protein 1 (RAP-1) is a targeted vaccine antigen for
Babesia bovis
and
Babesia bigemina
infections of cattle. The 60-kDa
B. bovis
RAP-1 is recognized by antibodies and T lymphocytes from cattle that recovered from infection and were immune to subsequent challenge. Immunization with native or recombinant protein was reported to reduce parasitemias in challenged animals. We recently reported that the NT domain of
B. bovis
RAP-1 contained immunodominant T-cell epitopes, whereas the repeat-rich CT domain was less immunostimulatory for T lymphocytes from cattle immune to
B. bovis.
The present study was therefore designed to test the hypothesis that the NT region of RAP-1, used as a vaccine with interleukin-12 and RIBI (catalog no. R-730; RIBI Immunochem Research, Inc., Hamilton, Mont. [now Corixa, Seattle, Wash.]) adjuvant to induce a type 1 response, would prime calves for antibody and T-helper cell responses comparable to or greater than those induced by full-length RAP-1 containing the C-terminal repeats. Furthermore, a type 1 immune response to RAP-1 was hypothesized to induce protection against challenge. Following four inoculations of either recombinant full-length RAP-1 or RAP-1 NT protein, RAP-1-specific immunoglobulin G (IgG) titers, T-lymphocyte proliferation, and gamma interferon production were similar. Similar numbers of NT region peptides were recognized. However, in spite of the presence of strong RAP-1-specific IgG and CD4
+
-T-lymphocyte responses that were recalled upon challenge, neither antigen stimulated a protective immune response. We conclude that successful priming of calves with recombinant RAP-1 and adjuvants that elicit strong Th1 cell and IgG responses is insufficient to protect calves against virulent
B. bovis
challenge.
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Details
- Title
- Stimulation of T-Helper Cell Gamma Interferon and Immunoglobulin G Responses Specific for Babesia bovis Rhoptry-Associated Protein 1 (RAP-1) or a RAP-1 Protein Lacking the Carboxy-Terminal Repeat Region Is Insufficient To Provide Protective Immunity against Virulent B. bovis Challenge
- Creators
- Junzo Norimine - Department of Veterinary Microbiology and PathologyJuan Mosqueda - Department of Veterinary Microbiology and PathologyCarlos Suarez - Department of Veterinary Microbiology and PathologyGuy H Palmer - Department of Veterinary Microbiology and PathologyTerry F McElwain - Department of Veterinary Microbiology and PathologyGabriel Mbassa - Department of Veterinary Microbiology and PathologyWendy C Brown - Department of Veterinary Microbiology and Pathology
- Publication Details
- Infection and immunity, Vol.71(9), pp.5021-5032
- Academic Unit
- Veterinary Microbiology and Pathology, Department of; Paul G. Allen School for Global Animal Health
- Publisher
- American Society for Microbiology
- Identifiers
- 99900546701601842
- Language
- English
- Resource Type
- Journal article