Journal article
Stimulation of in vitro ovarian estradiol-17β synthesis in the rainbow trout by the carbohydrate-poor protein fraction from sockeye salmon pituitary glands
The Journal of steroid biochemistry and molecular biology, Vol.39(4), pp.513-518
1991
Handle:
https://hdl.handle.net/2376/115868
PMID: 1911440
Abstract
The role of carbohydrate-poor (Con A I) and carbohydrate-rich (Con A II) pituitary protein fractions, isolated from sockeye salmon (
Oncorhynchus nerka), were investigated pertaining to
in vitro estradiol-17β (E
2) production by rainbow trout (
Oncorhynchus mykiss) ovarian follicles. During the early vitellogenic phase of the reproductive cycle, using defolliculated ovarian follicle preparations (outer epithelium-thecal layer absent), it was demonstrated that the Con A I fraction was capable of increasing E
2 production, in the presence of exogenous testosterone (T) as the substrate. Under similar conditions the Con A II fraction (containing the maturational gonadotropin) was inactive. However the Con A II fraction or T, separately, increased E
2 production by intact ovarian follicles, whereas the Con A I fraction did not. A mechanism proposed to explain the regulation of ovarian E
2 synthesis involves the Con A I fraction enhancing aromatase activity in granulosa cells permitting an increased conversion of T to E
2.
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Details
- Title
- Stimulation of in vitro ovarian estradiol-17β synthesis in the rainbow trout by the carbohydrate-poor protein fraction from sockeye salmon pituitary glands
- Creators
- James J NaglerDavid R Idler
- Publication Details
- The Journal of steroid biochemistry and molecular biology, Vol.39(4), pp.513-518
- Academic Unit
- UNKNOWN
- Publisher
- Elsevier Ltd
- Identifiers
- 99900547691301842
- Language
- English
- Resource Type
- Journal article