Journal article
Stimulation of microtubule dynamic turnover in living cells treated with okadaic acid
Cell motility and the cytoskeleton, Vol.35(1), pp.24-34
1996
Handle:
https://hdl.handle.net/2376/106193
PMID: 8874963
Abstract
We have examined the effects of okadaic acid, an inhibitor of protein phosphatases type 1 and 2A, on the dynamic instability behavior of individual microtubules in living cells. Addition of 1 μM okadaic acid to PtK1 epithelial cells induced ruffling of lamellar regions; after 50 min in okadaic acid, many cells were observed to round up. Confocal microscopy of okadaic acid‐treated cells stained with an antibody to tubulin showed that microtubules were more densely packed near the periphery of the rounded cells, and in many cells, a reduction in the density of microtubules near the microtubule‐organizing center was observed. The dynamic behavior of individual microtubules in cells previously injected with rhodamine‐labeled tubulin was quantified by tracking individual microtubules from image sequences. Microtubule dynamic turnover was markedly stimulated in cells treated with 1 μM okadaic acid for 50–60 min: The average rates of both microtubule growing and shortening increased, and the average duration of pause, or attenuation, a phase in which neither growth nor shortening could be detected, was significantly decreased. Further, okadaic acid induced an approximately twofold increase in the frequency of catastrophe transitions and a threefold decrease in the frequency of rescue transitions. Dynamicity, a measure of the net gain and loss of polymer at microtubule plus ends, increased nearly threefold in okadaic acid‐treated cells. These results demonstrate that microtubule turnover is stimulated in okadaic acid‐treated cells and suggest that phosphorylation of molecules which interact with microtubules may result in increased microtubule dynamic turnover in vivo. © 1996 Wiley‐Liss, Inc.
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Details
- Title
- Stimulation of microtubule dynamic turnover in living cells treated with okadaic acid
- Creators
- Eric SheldenPatricia Wadsworth
- Publication Details
- Cell motility and the cytoskeleton, Vol.35(1), pp.24-34
- Academic Unit
- Molecular Biosciences, School of
- Publisher
- Wiley Subscription Services, Inc., A Wiley Company; Hoboken
- Number of pages
- 11
- Identifiers
- 99900546711501842
- Language
- English
- Resource Type
- Journal article