Strawberry disease lesions in rainbow trout from southern Idaho are associated with DNA from a Rickettsia-like organism

Sonja J Lloyd; Scott E LaPatra; Kevin R Snekvik; Sophie St-Hilaire; Kenneth D Cain; Douglas R Call

doi:10.3354/dao01969

Back

Strawberry disease lesions in rainbow trout from southern Idaho are associated with DNA from a Rickettsia-like organism

Journal article

Open access

Peer reviewed

Strawberry disease lesions in rainbow trout from southern Idaho are associated with DNA from a Rickettsia-like organism

Sonja J Lloyd, Scott E LaPatra, Kevin R Snekvik, Sophie St-Hilaire, Kenneth D Cain and Douglas R Call

Diseases of aquatic organisms, Vol.82(2), pp.111-118

11/20/2008

DOI: https://doi.org/10.3354/dao01969

Handle:

https://hdl.handle.net/2376/105553

PMID: 19149374

Files and links (1)

url

https://doi.org/10.3354/dao01969View

Published (Version of record) Open

Abstract

Idaho - epidemiology

Skin Diseases - microbiology

Animals

DNA, Bacterial - genetics

DNA, Bacterial - isolation & purification

Fish Diseases - microbiology

Skin Diseases - veterinary

Fish Diseases - epidemiology

Rickettsiaceae - genetics

Phylogeny

Oncorhynchus mykiss

Rickettsiaceae - isolation & purification

Strawberry disease (SD) in the USA is a skin disorder of unknown etiology that occurs in rainbow trout Oncorhynchus mykiss and is characterized by bright red inflammatory lesions. To identify a candidate bacterial agent responsible for SD, we constructed 16S rDNA libraries from 7 SD lesion samples and 2 apparently healthy skin samples from SD-affected fish. A 16S rDNA sequence highly similar to members of the order Rickettsiales was present in 3 lesion libraries at 1%, 32% and 54% prevalence, but this sequence was not found in either healthy tissue library. Based on phylogenetic analysis, this Rickettsia-like organism (RLO) sequence is most closely related to 16S rDNA sequences of bacteria that may form a novel lineage within the Rickettsiales. We used nested PCR assays to screen 25 SD-affected fish for RLO or Flavobacterium psychrophilum DNA. Sixteen lesion samples were positive for the RLO sequence and 4 of the matched healthy samples were positive resulting in a significant association between SD lesions and presence of RLO DNA. While F. psychrophilum is reportedly associated with 'cold water strawberry disease' in the UK, we found no significant association between SD lesions and the presence of F. psychrophilum DNA. The statistical association between SD lesions and presence of RLO DNA is not proof of etiology, but these data suggest that RLO may play a role in SD in southern Idaho, USA.

Metrics

8 Record Views

Details

Title: Strawberry disease lesions in rainbow trout from southern Idaho are associated with DNA from a Rickettsia-like organism
Creators: Sonja J Lloyd - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164-7040, USA
Scott E LaPatra
Kevin R Snekvik
Sophie St-Hilaire
Kenneth D Cain
Douglas R Call
Publication Details: Diseases of aquatic organisms, Vol.82(2), pp.111-118
Academic Unit: Center for Reproductive Biology; Veterinary Microbiology and Pathology, Department of; Paul G. Allen School for Global Animal Health
Publisher: Germany
Identifiers: 99900546941601842
Language: English
Resource Type: Journal article