Journal article
Structure and genetic variability of envelope glycoproteins of two antigenic variants of caprine arthritis-encephalitis lentivirus
Journal of virology, Vol.65(11), pp.5744-5750
11/1991
Handle:
https://hdl.handle.net/2376/109090
PMCID: PMC250234
PMID: 1656067
Abstract
To define the structure of the caprine arthritis-encephalitis virus (CAEV) env gene and characterize genetic changes which occur during antigenic variation, we sequenced the env genes of CAEV-63 and CAEV-Co, two antigenic variants of CAEV defined by serum neutralization. The deduced primary translation product of the CAEV env gene consists of a 60- to 80-amino-acid signal peptide followed by an amino-terminal surface protein (SU) and a carboxy-terminal transmembrane protein (TM) separated by an Arg-Lys-Lys-Arg cleavage site. The signal peptide cleavage site was verified by amino-terminal amino acid sequencing of native CAEV-63 SU. In addition, immunoprecipitation of [35S]methionine-labeled CAEV-63 proteins by sera from goats immunized with recombinant vaccinia virus expressing the CAEV-63 env gene confirmed that antibodies induced by env-encoded recombinant proteins react specifically with native virion SU and TM. The env genes of CAEV-63 and CAEV-Co encode 28 conserved cysteines and 25 conserved potential N-linked glycosylation sites. Nucleotide sequence variability results in 62 amino acid changes and one deletion within the SU and 34 amino acid changes within the TM.
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Details
- Title
- Structure and genetic variability of envelope glycoproteins of two antigenic variants of caprine arthritis-encephalitis lentivirus
- Creators
- D P Knowles - USDA Agricultural Research Service Animal Disease Research Unit, College of Veterinary Medicine, Washington State University, Pullman 99164-7040W P Cheevers - USDA Agricultural Research Service Animal Disease Research Unit, College of Veterinary Medicine, Washington State University, Pullman 99164-7040T C McGuire - USDA Agricultural Research Service Animal Disease Research Unit, College of Veterinary Medicine, Washington State University, Pullman 99164-7040A L Brassfield - USDA Agricultural Research Service Animal Disease Research Unit, College of Veterinary Medicine, Washington State University, Pullman 99164-7040W G Harwood - USDA Agricultural Research Service Animal Disease Research Unit, College of Veterinary Medicine, Washington State University, Pullman 99164-7040T A Stem - USDA Agricultural Research Service Animal Disease Research Unit, College of Veterinary Medicine, Washington State University, Pullman 99164-7040
- Publication Details
- Journal of virology, Vol.65(11), pp.5744-5750
- Academic Unit
- Veterinary Microbiology and Pathology, Department of
- Identifiers
- 99900547149601842
- Language
- English
- Resource Type
- Journal article