Journal article
Study of a two-stage growth of DHA-producing marine algae Schizochytrium limacinum SR21 with shifting dissolved oxygen level
Applied microbiology and biotechnology, Vol.81(6), pp.1141-1148
01/2009
Handle:
https://hdl.handle.net/2376/102249
PMID: 18936938
Abstract
The culture protocol of Schizochytrium limacinum SR 21, a known docosahexaenoic acid (DHA) producing marine algae was modified in this study to better fit fermentation parameters, particularly control of dissolved oxygen (DO) to the known reproductive and growth biology of the microorganism. The cultures controlled at 50% DO saturation produced a cell density of 181 million cells/ml, whereas cultures with 10% DO produced only 98.4 million cells/ml. A fixed-agitation rate of 150 rpm resulted in an even lower density of 22.5 million cells/ml. Fifty percent DO saturation level led to a decreased pH, as well as a negative correlation with lipid accumulation, while low oxygen concentration was obligatory for lipid accumulation. This study indicated that high DO was preferred for the cells’ reproduction via release of zoospores. Thus, the culture of S. limacinum SR21 should be best divided into two stages: (1) a cell-number-increasing stage in which cell reproduction and cell number increase with little increase in the size and weight of each cell; and (2) a cell-size-increasing stage in which cells stop reproduction but cell size enlarges due to lipids accumulation. With such a protocol, the production of algae biomass and DHA was improved to levels of 37.9 g/L and 6.56 g/L, respectively. The two-stage culture process could be potentially used not only for omega-3 PUFA production, but also in other single cell oil (SCO)-producing processes, including biodiesel production from algae.
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Details
- Title
- Study of a two-stage growth of DHA-producing marine algae Schizochytrium limacinum SR21 with shifting dissolved oxygen level
- Creators
- Zhanyou Chi - Department of Biological Systems Engineering Washington State University Pullman WA 99164-6120 USAYan Liu - Biosystems and Agricultural Engineering Michigan State University East Lansing MI USACraig Frear - Department of Biological Systems Engineering Washington State University Pullman WA 99164-6120 USAShulin Chen - Department of Biological Systems Engineering Washington State University Pullman WA 99164-6120 USA
- Publication Details
- Applied microbiology and biotechnology, Vol.81(6), pp.1141-1148
- Academic Unit
- Biological Systems Engineering, Department of; Plant Pathology, Department of
- Publisher
- Springer-Verlag; Berlin/Heidelberg
- Identifiers
- 99900546524901842
- Language
- English
- Resource Type
- Journal article