Journal article
Studying pellet formation of a filamentous fungus Rhizopus oryzae to enhance organic acid production
Applied biochemistry and biotechnology, Vol.137(1), pp.689-701
04/2007
Handle:
https://hdl.handle.net/2376/104407
PMID: 18478426
Abstract
Using pelletized fungal biomass can effectively improve the fermentation performance for most of fugal strains. This article studied the effects of inoculum and medium compositions such as potato dextrose broth (PDB) as carbon source, soybean peptone, calcium carbonate, and metal ions on pellet formation of Rhizopus oryzae. It has been found that metal ions had significantly negative effects on pellet formation whereas soybean peptone had positive effects. In addition PDB and calcium carbonate were beneficial to R. oryzae for growing small smooth pellets during the culture. The study also demonstrated that an inoculum size of less than 1.5×109 spores/L had no significant influence on pellet formation. Thus, a new approach to form pellets has been developed using only PDB, soybean peptone, and calcium carbonate. Meanwhile, palletized fungal fermentation significantly enhanced organic acid production. Lactic acid concentration reached 65.0 g/L in 30 h using pelletized R. oryzae NRRL 395, and fumeric acid concentration reached 31.0 g/L in 96 h using pelletized R. oryzae ATCC 20344.
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Details
- Title
- Studying pellet formation of a filamentous fungus Rhizopus oryzae to enhance organic acid production
- Creators
- Wei Liao - Department of Biological Systems Engineering, and Center for Bioenergy and Bioproducts Washington State University 99163 Pullman WAYan Liu - Department of Biological Systems Engineering, and Center for Bioenergy and Bioproducts Washington State University 99163 Pullman WAShulin Chen - Department of Biological Systems Engineering, and Center for Bioenergy and Bioproducts Washington State University 99163 Pullman WA
- Publication Details
- Applied biochemistry and biotechnology, Vol.137(1), pp.689-701
- Academic Unit
- Biological Systems Engineering, Department of; Plant Pathology, Department of
- Publisher
- Humana Press; Totowa
- Identifiers
- 99900546521201842
- Language
- English
- Resource Type
- Journal article