Journal article
Stylet penetration of Cacopsylla pyri; an electrical penetration graph (EPG) study
Journal of insect physiology, Vol.57(10), pp.1407-1419
2011
Handle:
https://hdl.handle.net/2376/113004
PMID: 21802423
Abstract
[Display omitted]
► EPG is the best technique to study plant penetration activities by
Cacopsylla pyri. ► Each EPG waveform was histologically correlated to tissues reached by stylet tips. ► More than one waveform occurred in the same tissue. ► No brief intracellular punctures were observed during stylet penetration of
C. pyri.
Detailed information on plant penetration activities by pear psylla
Cacopsylla pyri L. (Hemiptera Psyllidae) is essential to study phytoplasma transmission of “
Candidatus Phytoplasma pyri” responsible of pear decline disease (PD) and to trace and evaluate resistant traits in new pear tree selections for advanced breeding programs. The electrical penetration graph technique or (full) EPG may relevantly contribute to this knowledge.
C. pyri EPG waveforms were characterized on basis of amplitude, frequency, voltage level, and electrical origin. Additionally, stylet tracks and the putative location of stylet tips in the plant tissue were histologically related to EPG waveforms by light and transmission electron microscopy observations after stylectomy. More than one waveform occurred in the same tissue: PA, PB, PC1 and PC2 were all detected in the mesophyll, and PE1 and PE2 were both recorded in the phloem. Waveform PE1 was always preceded by transient waveform PD, as previously described in other psyllids. Interestingly, no brief intracellular punctures (potential drop waveforms) were observed during plant penetration, opposite of what is usually recorded in aphids and other Sternorrhyncha.
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Details
- Title
- Stylet penetration of Cacopsylla pyri; an electrical penetration graph (EPG) study
- Creators
- Stefano Civolani - Department of Biology and Evolution, University of Ferrara, via L. Borsari 46, 44100 Ferrara, ItalyMarilena Leis - Department of Biology and Evolution, University of Ferrara, via L. Borsari 46, 44100 Ferrara, ItalyGilberto Grandi - Department of Biology and Evolution, University of Ferrara, via L. Borsari 46, 44100 Ferrara, ItalyElisa Garzo - Instituto de Ciencias Agrarias, Centro de Ciencias Medioambientales, CSIC Serrano 115bis, 28006 Madrid, SpainEdison Pasqualini - Department of Agroenvironmental Sciences and Technologies, University of Bologna, via G. Fanin 44, 40127 Bologna, ItalyStefano Musacchi - Department of Fruit Tree and Woody Plant Science, University of Bologna, via G. Fanin 46, 40127 Bologna, ItalyMilvia Chicca - Department of Biology and Evolution, University of Ferrara, via L. Borsari 46, 44100 Ferrara, ItalyGiuseppe Castaldelli - Department of Biology and Evolution, University of Ferrara, via L. Borsari 46, 44100 Ferrara, ItalyRemigio Rossi - Department of Biology and Evolution, University of Ferrara, via L. Borsari 46, 44100 Ferrara, ItalyW Freddy Tjallingii - Laboratory of Entomology – Wageningen University/EPG Systems, Droevendaalsesteeg 1, 6708 PB Wageningen, The Netherlands
- Publication Details
- Journal of insect physiology, Vol.57(10), pp.1407-1419
- Academic Unit
- Horticulture, Department of
- Publisher
- Elsevier Ltd
- Identifiers
- 99900547622701842
- Language
- English
- Resource Type
- Journal article