Subunit configuration of heteromeric cone cyclic nucleotide-gated channels

Changhong Peng; Elizabeth D Rich; Michael D Varnum

doi:10.1016/S0896-6273(04)00225-9

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Subunit configuration of heteromeric cone cyclic nucleotide-gated channels

Journal article

Open access

Peer reviewed

Subunit configuration of heteromeric cone cyclic nucleotide-gated channels

Changhong Peng, Elizabeth D Rich and Michael D Varnum

Neuron (Cambridge, Mass.), Vol.42(3), pp.401-410

05/13/2004

DOI: https://doi.org/10.1016/S0896-6273(04)00225-9

Handle:

https://hdl.handle.net/2376/107790

PMID: 15134637

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Abstract

Cyclic GMP - pharmacology

Ion Channels - biosynthesis

Humans

Xenopus laevis

Ion Channels - genetics

Protein Subunits - biosynthesis

Dose-Response Relationship, Drug

Diltiazem - pharmacology

Animals

Retinal Cone Photoreceptor Cells - chemistry

Retinal Cone Photoreceptor Cells - drug effects

Female

Protein Subunits - chemistry

Cyclic Nucleotide-Gated Cation Channels

Ion Channels - chemistry

Retinal Cone Photoreceptor Cells - metabolism

Protein Subunits - genetics

Cone photoreceptor cyclic nucleotide-gated (CNG) channels are thought to be tetrameric assemblies of CNGB3 (B3) and CNGA3 (A3) subunits. We have used functional and biochemical approaches to investigate the stoichiometry and arrangement of these subunits in recombinant channels. First, tandem dimers of linked subunits were used to constrain the order of CNGB3 and CNGA3 subunits; the properties of channels formed by B3/B3+A3/A3 dimers, or A3/B3+B3/A3 dimers, closely resembled those of channels arising from B3+A3 monomers. Functional markers in B3/B3 (or A3/A3) dimers confirmed that both B3 subunits (and both A3 subunits) gained membership into the pore-forming tetramer and that like subunits were positioned adjacent to each other. Second, chemical crosslinking and co-immunoprecipitation studies using epitope-tagged monomer subunits both demonstrated the presence of two CNGB3 subunits in cone channels. Together, these data support a preferred subunit arrangement for cone CNG channels (B3-B3-A3-A3) that is distinct from the 3A:1B configuration of rod channels.

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Title: Subunit configuration of heteromeric cone cyclic nucleotide-gated channels
Creators: Changhong Peng - Department of Veterinary and Comparative Anatomy, Washington State University, P.O. Box 646520, Pullman, WA 99164, USA
Elizabeth D Rich
Michael D Varnum
Publication Details: Neuron (Cambridge, Mass.), Vol.42(3), pp.401-410
Academic Unit: Integrative Physiology and Neuroscience, Department of
Publisher: United States
Grant note: EY 12836 / NEI NIH HHS
Identifiers: 99900547243901842
Language: English
Resource Type: Journal article