Suites of terpene synthases explain differential terpenoid production in ginger and turmeric tissues

Hyun Jo Koo; David R Gang

doi:10.1371/journal.pone.0051481

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Suites of terpene synthases explain differential terpenoid production in ginger and turmeric tissues

Journal article

Open access

Suites of terpene synthases explain differential terpenoid production in ginger and turmeric tissues

Hyun Jo Koo and David R Gang

PloS one, Vol.7(12), pp.e51481-e51481

2012

DOI: https://doi.org/10.1371/journal.pone.0051481

Handle:

https://hdl.handle.net/2376/111008

PMCID: PMC3525583

PMID: 23272109

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https://doi.org/10.1371/journal.pone.0051481View

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Abstract

Alkyl and Aryl Transferases - chemistry

Cell Line

Plant Extracts - chemistry

Gas Chromatography-Mass Spectrometry - methods

Temperature

Models, Chemical

Plant Extracts - pharmacology

Humans

Molecular Conformation

Ginger - metabolism

DNA Primers - genetics

Phylogeny

Curcuma - metabolism

Terpenes - chemistry

Alkyl and Aryl Transferases - physiology

Codon

Rhizome - chemistry

Cloning, Molecular

Escherichia coli - metabolism

Cell Line, Tumor

DNA, Complementary - metabolism

Expressed Sequence Tags

Yeasts - metabolism

The essential oils of ginger (Zingiber officinale) and turmeric (Curcuma longa) contain a large variety of terpenoids, some of which possess anticancer, antiulcer, and antioxidant properties. Despite their importance, only four terpene synthases have been identified from the Zingiberaceae family: (+)-germacrene D synthase and (S)-β-bisabolene synthase from ginger rhizome, and α-humulene synthase and β-eudesmol synthase from shampoo ginger (Zingiber zerumbet) rhizome. We report the identification of 25 mono- and 18 sesquiterpene synthases from ginger and turmeric, with 13 and 11, respectively, being functionally characterized. Novel terpene synthases, (-)-caryolan-1-ol synthase and α-zingiberene/β-sesquiphellandrene synthase, which is responsible for formation of the major sesquiterpenoids in ginger and turmeric rhizomes, were also discovered. These suites of enzymes are responsible for formation of the majority of the terpenoids present in these two plants. Structures of several were modeled, and a comparison of sets of paralogs suggests how the terpene synthases in ginger and turmeric evolved. The most abundant and most important sesquiterpenoids in turmeric rhizomes, (+)-α-turmerone and (+)-β-turmerone, are produced from (-)-α-zingiberene and (-)-β-sesquiphellandrene, respectively, via α-zingiberene/β-sesquiphellandrene oxidase and a still unidentified dehydrogenase.

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Title: Suites of terpene synthases explain differential terpenoid production in ginger and turmeric tissues
Creators: Hyun Jo Koo - Department of Plant Sciences and Bio5 Institute, The University of Arizona, Tucson, Arizona, United States of America
David R Gang
Publication Details: PloS one, Vol.7(12), pp.e51481-e51481
Academic Unit: Biological Chemistry, Institute of
Publisher: United States
Identifiers: 99900547283901842
Language: English
Resource Type: Journal article