Journal article
Sulfinylated Azadecalins act as functional mimics of a pollen germination stimulant in Arabidopsis pistils
The Plant journal : for cell and molecular biology, Vol.68(5), pp.800-815
12/2011
Handle:
https://hdl.handle.net/2376/109736
PMCID: PMC3225508
PMID: 21801250
Abstract
Polarized cell elongation is triggered by small molecule cues during development of diverse organisms. During plant reproduction, pollen interactions with the stigma result in the polar outgrowth of a pollen tube, which delivers sperm cells to the female gametophyte to effect double fertilization. In many plants, pistils stimulate pollen germination. However, in Arabidopsis, the effect of pistils on pollen germination and the pistil factors that stimulate pollen germination remain poorly characterized. Here, we demonstrate that stigma, style, and ovules in Arabidopsis pistils stimulate pollen germination. We isolated an Arabidopsis pistil extract fraction that stimulates Arabidopsis pollen germination, and employed ultrahigh resolution ESI FT-ICR and MS/MS techniques to accurately determine the mass (202.126 daltons) of a compound that is specifically present in this pistil extract fraction. Using the molecular formula (C
10
H
19
NOS) and tandem mass spectral fragmentation patterns of the
m/z
(mass to charge ratio) 202.126 ion, we postulated chemical structures, devised protocols, synthesized
N
-Methanesulfinyl 1- and 2-azadecalins that are close structural mimics of the
m/z
202.126 ion, and showed that they are sufficient to stimulate Arabidopsis pollen germination
in vitro
(30 µM stimulated ~50% germination) and elicit accession-specific response. Although
N
-Methanesulfinyl 2-azadecalin stimulated pollen germination in three species of Lineage I of Brassicaceae, it did not induce a germination response in
Sisymbrium irio
(Lineage II of Brassicaceae) and tobacco, indicating that activity of the compound is not random. Our results show that Arabidopsis pistils promote germination by producing azadecalin-like molecules to ensure rapid fertilization by the appropriate pollen.
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Details
- Title
- Sulfinylated Azadecalins act as functional mimics of a pollen germination stimulant in Arabidopsis pistils
- Creators
- Yuan Qin - The School of Plant Sciences, University of Arizona, Tucson, AZ 85721, USARonald J Wysocki - Department of Chemistry and Biochemistry, University of Arizona, Tucson, AZ 85721Arpad Somogyi - Department of Chemistry and Biochemistry, University of Arizona, Tucson, AZ 85721Yelena Feinstein - Arizona Proteomics Consortium, University of Arizona, Tucson, AZ 85721, USAJessica Y Franco - The School of Plant Sciences, University of Arizona, Tucson, AZ 85721, USATatsuya Tsukamoto - The School of Plant Sciences, University of Arizona, Tucson, AZ 85721, USADamayanthi Dunatunga - The School of Plant Sciences, University of Arizona, Tucson, AZ 85721, USAClara Levy - Department of Molecular Biology, Cell Biology, and Biochemistry, Brown University, Providence, RI 02912, U.S.ASteven Smith - The School of Plant Sciences, University of Arizona, Tucson, AZ 85721, USARobert Simpson - Pima Community College, Tucson, AZ 85709, USADavid Gang - The School of Plant Sciences, University of Arizona, Tucson, AZ 85721, USAMark A Johnson - Department of Molecular Biology, Cell Biology, and Biochemistry, Brown University, Providence, RI 02912, U.S.ARavishankar Palanivelu - The School of Plant Sciences, University of Arizona, Tucson, AZ 85721, USA
- Publication Details
- The Plant journal : for cell and molecular biology, Vol.68(5), pp.800-815
- Academic Unit
- Plant Pathology, Department of; Biological Chemistry, Institute of
- Grant note
- S10 RR022384-01 || RR / National Center for Research Resources : NCRR
- Identifiers
- 99900547050801842
- Language
- English
- Resource Type
- Journal article