Sulforaphane induces SLPI secretion in the nasal mucosa

Megan Meyer; Matthew J Kesic; John Clarke; Emily Ho; Rosalia C.M Simmen; David Diaz-Sanchez; Terry L Noah; Ilona Jaspers

doi:10.1016/j.rmed.2012.11.006

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Sulforaphane induces SLPI secretion in the nasal mucosa

Journal article

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Sulforaphane induces SLPI secretion in the nasal mucosa

Megan Meyer, Matthew J Kesic, John Clarke, Emily Ho, Rosalia C.M Simmen, David Diaz-Sanchez, Terry L Noah and Ilona Jaspers

Respiratory medicine, Vol.107(3), pp.472-475

03/2013

DOI: https://doi.org/10.1016/j.rmed.2012.11.006

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https://hdl.handle.net/2376/108305

PMCID: PMC3640824

PMID: 23195333

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Abstract

SLPI

Sulforaphane

Nasal mucosa

Cells lining the respiratory tract are equipped with mechanisms that dampen the effects of oxidative stress. Nuclear factor (erythroid-derived 2)-like 2 (Nrf2) is a mediator involved in regulating oxidative stress. Recent data indicate Nrf2 also controls expression of secretory leukocyte protease inhibitor (SLPI). Sulforaphane (SFN), an isothiocyanate found in cruciferous vegetables, enhances Nrf2 activity. Therefore, we hypothesized that SFN supplementation induces SLPI secretion in the nasal mucosa in an Nrf2 dependent manner. Healthy nonsmoking adults ingested SFN-containing broccoli shake homogenate (BSH) for 3 consecutive days. Nasal lavage fluid (NLF) was collected before and after BSH ingestion and analyzed for SLPI protein levels. In follow up in vitro experiments, differentiated primary nasal epithelial cells were used to evaluate the relationship between SFN, Nrf2, and SLPI. Epithelial cells were transduced with Nrf2-specific shRNA to examine the regulatory role of Nrf2 on SLPI expression. Supplementation with BSH significantly increased SLPI levels in NLF. SFN supplementation in vitro significantly enhanced SLPI secretion and these effects were significantly decreased in cells transduced with Nrf2-specific shRNA. Our data support a relationship between nutritional supplementation, Nrf2 activation, and SLPI secretion. Therefore, ingestion of SFN-containing foods has therapeutic potential to augment SLPI expression in the nasal mucosa.

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Title: Sulforaphane induces SLPI secretion in the nasal mucosa
Creators: Megan Meyer - Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA
Matthew J Kesic - Center for Environmental Medicine, Asthma and Lung Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA
John Clarke - School of Biological & Population Health Sciences, Oregon State University, USA
Emily Ho - School of Biological & Population Health Sciences, Oregon State University, USA
Rosalia C.M Simmen - Department of Physiology & Biophysics, University of Arkansas for Medical Sciences, Little Rock, AR, USA
David Diaz-Sanchez - U.S. Environmental Protection Agency, Chapel Hill, NC, USA
Terry L Noah - Center for Environmental Medicine, Asthma and Lung Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA
Ilona Jaspers - Center for Environmental Medicine, Asthma and Lung Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA
Publication Details: Respiratory medicine, Vol.107(3), pp.472-475
Academic Unit: Pharmaceutical Sciences, Department of
Publisher: Elsevier Ltd
Identifiers: 99900546880801842
Language: English
Resource Type: Journal article