Journal article
Summary of workshop findings for antibodies reacting with porcine T-cells and activation antigens: results from the Second International Swine CD Workshop
Veterinary immunology and immunopathology, Vol.60(3), pp.251-260
1998
Handle:
https://hdl.handle.net/2376/117678
PMID: 9589563
Abstract
After initial evaluation of the 176 new and 19 control monoclonal antibodies (mAb) submitted to the Second International Swine CD Workshop, 57 were assigned to the T-cell/activation marker subgroup. These 57 mAb were further analyzed using flow cytometry on whole blood lymphocytes, splenocytes, Peyer's patch lymphocytes, in vitro cell lines, broncho-alveolar lavage cells, Con A and PHA blasts, fetal cell populations, and by 2-color flow cytometry against mAb to porcine CD2, CD4, and CD8. Finally, the molecular weights of the target antigens were characterized when possible. As a result of these analyses, 23 mAb were distributed into 7 CD clusters. Newly confirmed mAb assignments included: two CD2; one CD4; two CD5; one wCD6; and one wCD25. Three new mAb were found that reacted with wCD8, one of which defined a new epitope, wCD8c. For the first time, mAb against porcine CD3 were identified, including 6 mAb that reacted with three different epitopes. Several new mAb reacted with antigens whose expression varied depending on the activation state of the test cell. These will require further characterization in order to assign a CD number.
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Details
- Title
- Summary of workshop findings for antibodies reacting with porcine T-cells and activation antigens: results from the Second International Swine CD Workshop
- Creators
- M.D Pescovitz - Indiana University, Department of Surgery and Microbiology/Immunology, Indianapolis, IN 46202, USAB.K Book - Indiana University, Department of Surgery and Microbiology/Immunology, Indianapolis, IN 46202, USAB Aasted - Royal Veterinary and Agricultural University, Frederiksberg C, DenmarkJ Dominguez - Centro de Investigaciones en Sanidad Animal, INIA, Valdeolmos, Madrid, SpainA Ezquerra - Centro de Investigaciones en Sanidad Animal, INIA, Valdeolmos, Madrid, SpainI Trebichavsky - Institute Microbiology, Prague, Czech RepublicB Novikov - All-Russian Research Institute of Veterinary Virology and Microbiology, Vlasimizskaya obl., Russian FederationI Valpotic - Department of Biology, Veterinary Faculty, University of Zagreb, Zagreb, CroatiaL Sver - Department of Biology, Veterinary Faculty, University of Zagreb, Zagreb, CroatiaJ Nielsen - Danish Veterinary Institute for Virus Research, Lindholm, Kalvehave, DenmarkS Arn - Transplantation Biology Research Center, Massachusetts General Hospital, Charlestown, MA, USAD.H Sachs - Transplantation Biology Research Center, Massachusetts General Hospital, Charlestown, MA, USAJ.K Lunney - Immunology and Disease Resistance Laboratory, LPSI, ARS, USDA, Beltsville, MD, USAP.C Boyd - Immunology and Disease Resistance Laboratory, LPSI, ARS, USDA, Beltsville, MD, USAJ Walker - Center for Animal Biotechnology, University of Melbourne, Victoria, AustraliaR Lee - Center for Animal Biotechnology, University of Melbourne, Victoria, AustraliaWilliam Davis - Washington State University, Pullman, WA, USAI.R Barbosa - Washington State University, Pullman, WA, USAF Zuckermann - Department of Veterinary Pathology, University of Illinois, Urbana, IL, USAA Saalmüller - Federal Research Center for Virus Diseases of Animals, Tübingen, Germany
- Publication Details
- Veterinary immunology and immunopathology, Vol.60(3), pp.251-260
- Academic Unit
- Medical Education and Clinical Science, Department of; Veterinary Microbiology and Pathology, Department of
- Publisher
- Elsevier B.V
- Identifiers
- 99900548142501842
- Language
- English
- Resource Type
- Journal article